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  6. Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 

Cat. No.: HY-K0010
Manual COA SDS Technical Support

MCE Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) is a blend of 6 pan-protease inhibitors for protection of protein integrity.

Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)
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1357 Publications Citing Use of MCE Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

WB
IP

    Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Apr 4;23(1):70.  [Abstract]

    Western blot analysis of EVs markers and endoplasmic reticulum marker in whole cell lysates and EVs.

    Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 Aug 20.  [Abstract]

    The cels were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
    Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

    Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MedChemExpress. Usage Cited in: Neuro Oncol. 2024 Aug 2:noae147.  [Abstract]

    Co-immunoprecipitation of TNFR1 or VASN in T4121 and WF19 GSCs and immunoblotting for TNFR1 and VASN. IgG was used as the control antibody for IP.

    MCE Biological Validation

    WB
    IP

      Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MCE. Usage Cited in: Mol Cancer. 2024 Apr 4;23(1):70.  [Abstract]

      Customer Validation
      Western blot analysis of EVs markers and endoplasmic reticulum marker in whole cell lysates and EVs.

      Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MCE. Usage Cited in: Cell Mol Immunol. 2024 Aug 20.  [Abstract]

      Customer Validation
      The cels were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
      Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

      Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) purchased from MCE. Usage Cited in: Neuro Oncol. 2024 Aug 2:noae147.  [Abstract]

      Customer Validation
      Co-immunoprecipitation of TNFR1 or VASN in T4121 and WF19 GSCs and immunoblotting for TNFR1 and VASN. IgG was used as the control antibody for IP.

      • Description

      • Price Comparison

      • Storage

      • Components

      • Documentation

      Description
      & Advantages

      MCE Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.

      MCE Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.

      MCE Protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.

      Price Comparison
      Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) Price Comparison
      Storage

      -20°C, 2 years
      Components
      Cat. No. Product Name Package
      HY-K0010-1 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL
      HY-K0010-10 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL × 10
      HY-K0010-50 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL × 50
      HY-K0010-100 mL Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) 1 mL × 100
      Documentation

      Manual (1020 KB) SDS (4582 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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