2. Academic Validation
  3. Fucosylation of hemocyanin is critical for antibacterial immunity in Penaeus vannamei

Fucosylation of hemocyanin is critical for antibacterial immunity in Penaeus vannamei

  • Fish Shellfish Immunol. 2025 Aug:163:110406. doi: 10.1016/j.fsi.2025.110406.
Mingming Jiang 1,  Yiqi Liu 1,  Jude Juventus Aweya 2,  Shuaiqi Liang 1,  Junyang Zhou 1,  Muhammad Tayyab 1,  Yongzhen Zhao 3,  Qingyun Liu 3,  Yueling Zhang 4,  Zhihong Zheng 5
Affiliations

Affiliations

  • 1 Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, 515063, China.
  • 2 Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, 515063, China; Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada; The Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, MB, Canada.
  • 3 Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China.
  • 4 Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, 515063, China. Electronic address: zhangyl@stu.edu.cn.
  • 5 Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, 515063, China. Electronic address: zhengzh@stu.edu.cn.
PMID: 40350103 DOI: 10.1016/j.fsi.2025.110406
Abstract

Posttranslational modifications (PTMs) play a pivotal role in expanding the function of immune related proteins, especially during pathogen infections. However, the precise impact of PTMs on the functional diversity of proteins such as respiratory glycoproteins and hemocyanins remains incompletely understood. In this study, we investigated the fucosylation modification of Penaeus vannamei hemocyanin (PvHMC) and its impact on Antibacterial immunity. Our findings reveal that PvHMC underwent fucosylation, mediated by Penaeus vannamei fucosyltransferase 8/10 (PvFUT8/10), which enhances its Antibacterial immunity and binding ability against Gram-negative bacteria. Conversely, defucosylation of PvHMC, catalyzed by Penaeus vannamei fucosidase (PvAFU) weakens its Antibacterial activity in vitro. These results highlight the regulatory role of fucosylation in modulating the Antibacterial function of PvHMC in shrimp.

Keywords

Antibacterial immunity; Fucosidase; Fucosylation; Fucosyltransferase; Penaeus vannamei, hemocyanin; Posttranslational modifications (PTMs).

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