1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes

Ubiquitination is a tightly controlled three-step multienzyme cascades. A ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin ligase (E3) act in a concerted manner to form a covalent bond between ubiquitin and its substrate protein, and thereby write the ubiquitin code. In detail, this process is initiated by a family of mechanistically and structurally-related E1 enzymes. The E1s serve to activate ubiquitin/UBLs through C-terminal adenylation and thiol transfer and to coordinate the utilization of ubiquitin/UBLs in specific downstream pathways by charging cognate E2 enzymes, which then interact with the downstream ubiquitylation machinery to coordinate modification of the target.

Cat. No. Product Name / Synonyms Species Source
  • HY-P73485
    XIAP Protein, Human (Avi)

    E3 ubiquitin-protein ligase XIAP; ILP; IAP-3; XIAP; API3; BIRC4

    Human E. coli
    XIAP protein is a multifunctional regulator that controls apoptosis, inflammation, immunity, copper homeostasis, and various signaling pathways. As a caspase inhibitor, it blocks CASP3 and CASP7 substrate access, keeping CASP9 inactive. XIAP Protein, Human (Avi) is the recombinant human-derived XIAP protein, expressed by E. coli , with C-Avi labeled tag. The total length of XIAP Protein, Human (Avi) is 236 a.a., with molecular weight of ~29.1 kDa.
  • HY-P73537
    XIAP Protein, Human (His)

    E3 ubiquitin-protein ligase XIAP; ILP; IAP-3; XIAP; API3; BIRC4

    Human E. coli
    XIAP protein is a multifunctional regulator that controls apoptosis, inflammation, immunity, copper homeostasis, and various signaling pathways. As a caspase inhibitor, it blocks CASP3 and CASP7 substrate access, keeping CASP9 inactive. XIAP Protein, Human (His) is the recombinant human-derived XIAP protein, expressed by E. coli , with N-His labeled tag. The total length of XIAP Protein, Human (His) is 105 a.a., with molecular weight of ~14.8 kDa.
  • HY-P74485
    UBA1 Protein, Human (sf9, His-GST)

    Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; UBA1; A1S9T; UBE1

    Human Sf9 insect cells
    The UBA1 protein initiates ubiquitin conjugation, marking proteins for degradation in the ubiquitin-proteasome system. UBA1 Protein, Human (sf9, His-GST) is the recombinant human-derived UBA1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of UBA1 Protein, Human (sf9, His-GST) is 1057 a.a., with molecular weight of ~130 kDa.
  • HY-P71399
    UBE2D1 Protein, Human (GST)

    Ubiquitin-conjugating enzyme E2 D1; Stimulator of Fe transport; SFT; UBC4/5 homolog; UbcH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1; SFT; UBC5A; UBCH5; UBCH5A

    Human E. coli
    UBE2D1 Protein, crucial in cellular processes, accepts ubiquitin from the E1 complex, preferentially catalyzing 'Lys-48'-linked polyubiquitination in vitro, showcasing versatility. It mediates selective degradation of short-lived and aberrant proteins and participates in E6/E6-AP-induced p53/TP53 ubiquitination. UBE2D1 facilitates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6, and TRIM63/MURF1. It ubiquitinates STUB1-associated HSP90AB1 in vitro, highlighting involvement in cellular pathways. Lacking inherent specificity for any ubiquitin lysine residue, UBE2D1's dynamic nature in ubiquitin modification processes is notable. Its essential role in viral activation of IRF3 and mediation of CYP3A4 polyubiquitination emphasizes multifaceted contributions to cellular function. UBE2D1 Protein, Human (GST) is the recombinant human-derived UBE2D1 protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2D1 Protein, Human (GST) is 147 a.a., with molecular weight of ~40.0 kDa.
  • HY-P71395
    UBA5 Protein, Human (His)

    Ubiquitin-like modifier-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1; UBE1DC1; UBA5

    Human E. coli
    The UBA5 protein activates UFM1 in ufmylation, linking the C-terminal glycine residue of UFM1 to a cysteine residue in E1. UBA5 Protein, Human (His) is the recombinant human-derived UBA5 protein, expressed by E. coli , with N-6*His labeled tag.
  • HY-P70998
    UBE2D3 Protein, Human

    Ubiquitin-conjugating enzyme E2 D3; Ubiquitin carrier protein D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; Ubiquitin-protein ligase D3; UBE2D3 and UBCH5C.

    Human E. coli
    UBE2D3 is able to accept ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfer it to substrates, often promoting its degradation by the proteasome. UBE2D3 Protein, Human is the recombinant human-derived UBE2D3 protein, expressed by E. coli , with tag free. The total length of UBE2D3 Protein, Human is 147 a.a., with molecular weight of ~15.0 kDa.
  • HY-P71020
    UBE2L6 Protein, Human (His)

    Ubiquitin/ISG15-Conjugating Enzyme E2 L6; Retinoic Acid-Induced Gene B Protein; RIG-B; UbcH8; Ubiquitin Carrier Protein L6; Ubiquitin-Protein Ligase L6; UBE2L6; UBCH8

    Human E. coli
    The UBE2L6 protein plays a key role in cell regulation by catalyzing the covalent attachment of ubiquitin or ISG15 to other proteins. Notably, it plays a role in E6/E6-AP-induced ubiquitination of p53/TP53, a core process in regulating cellular responses to stress and DNA damage. UBE2L6 Protein, Human (His) is the recombinant human-derived UBE2L6 protein, expressed by E. coli , with C-6*His labeled tag. The total length of UBE2L6 Protein, Human (His) is 153 a.a., with molecular weight of ~17.0 kDa.
  • HY-P71397
    UBE2A Protein, Human (GST-His)

    Ubiquitin-Conjugating Enzyme E2 A; RAD6 Homolog A; HR6A; hHR6A; Ubiquitin Carrier Protein A; Ubiquitin-Protein Ligase A; UBE2A; RAD6A

    Human E. coli
    UBE2A cooperates with the E3 enzyme BRE1 to promote the attachment of ubiquitin to proteins, specifically histone H2B at "Lys-120", forming H2BK120ub1. This modification serves as a tag for transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is critical for the formation of H3K4me and H3K79me. UBE2A Protein, Human (GST-His) is the recombinant human-derived UBE2A protein, expressed by E. coli , with N-6*His, N-GST labeled tag. The total length of UBE2A Protein, Human (GST-His) is 152 a.a., with molecular weight of ~52.0 kDa.
  • HY-P71401
    UBE2G2 Protein, Human (GST)

    Ubiquitin-Conjugating Enzyme E2 G2; Ubiquitin Carrier Protein G2; Ubiquitin-Protein Ligase G2; UBE2G2

    Human E. coli
    UBE2G2 Protein, a vital ubiquitination component, accepts ubiquitin from the E1 complex, catalyzing its covalent attachment to various proteins, with a preference for 'Lys-48'-linked polyubiquitination. Its specificity in shaping ubiquitin chains underscores its role in cellular processes. Intricately associated with endoplasmic reticulum-associated degradation (ERAD), UBE2G2 maintains cellular homeostasis by regulating protein turnover. Indispensable for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase, it facilitates subsequent proteasomal degradation. UBE2G2 Protein, Human (GST) is the recombinant human-derived UBE2G2 protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2G2 Protein, Human (GST) is 165 a.a., with molecular weight of ~43.0 kDa.
  • HY-P71402
    UBE2H Protein, Human (GST)

    Ubiquitin-Conjugating Enzyme E2 H; UbcH2; Ubiquitin Carrier Protein H; Ubiquitin-Conjugating Enzyme E2-20K; Ubiquitin-Protein Ligase H; UBE2H

    Human E. coli
    UBE2H Protein, a crucial participant in cellular ubiquitination, transfers ubiquitin from the E1 complex to various proteins, including MAEA of the CTLH E3 ubiquitin-protein ligase complex. Versatile in vitro, UBE2H catalyzes both 'Lys-11'- and 'Lys-48'-linked polyubiquitination, showcasing its involvement in diverse ubiquitin-related processes. Notably, it demonstrates the capability to ubiquitinate histone H2A in vitro. UBE2H Protein, Human (GST) is the recombinant human-derived UBE2H protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2H Protein, Human (GST) is 183 a.a., with molecular weight of ~50.0 kDa.
  • HY-P71403
    UBE2I Protein, Human (GST)

    SUMO-Conjugating Enzyme UBC9; SUMO-Protein Ligase; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Conjugating Enzyme E2 I; Ubiquitin-Protein Ligase I; p18; UBE2I; UBC9; UBCE9

    Human E. coli
    UBE2I Protein, a key participant in protein sumoylation, accepts ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4, and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. Teaming up with E3 ligases like RANBP2, CBX4, and ZNF451, UBE2I catalyzes the covalent attachment of these SUMO proteins to target proteins, including critical substrates FOXL2 and KAT5. Its enzymatic activity extends to forming poly-SUMO chains, influencing diverse cellular processes and playing a pivotal role in specific protein modifications, such as sumoylation of p53/TP53 at 'Lys-386' and ERCC6. UBE2I Protein, Human (GST) is the recombinant human-derived UBE2I protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2I Protein, Human (GST) is 158 a.a., with molecular weight of ~40.0 kDa.
  • HY-P71405
    UBE2K Protein, Human (GST)

    Ubiquitin-Conjugating Enzyme E2 K; Huntingtin-Interacting Protein 2; HIP-2; Ubiquitin Carrier Protein; Ubiquitin-Conjugating Enzyme E2-25 kDa; Ubiquitin-Conjugating Enzyme E2(25K); Ubiquitin-Conjugating Enzyme E2-25K; Ubiquitin-Protein Ligase; UBE2K; HIP2; LIG

    Human E. coli
    UBE2K protein is an important component of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme, which is critical for attaching ubiquitin to different substrates. In vitro, UBE2K catalyzes "Lys-48" linked polyubiquitin chains, specifically in the presence or absence of the BRCA1-BARD1 E3 ligase complex. UBE2K Protein, Human (GST) is the recombinant human-derived UBE2K protein, expressed by E. coli , with N-GST labeled tag.
  • HY-P71406
    UBE2M Protein, Human

    NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M; UBC12; UBE2M;

    Human E. coli
    UBE2M Protein is a crucial mediator in the ubiquitin-like protein NEDD8 conjugation pathway. It accepts NEDD8 from the UBA3-NAE1 E1 complex, covalently attaching it to target proteins like CUL1, CUL2, CUL3, and CUL4, particularly interacting with the E3 ubiquitin ligase RBX1. This specificity in neddylating specific targets suggests a pivotal role in regulating cellular processes, notably contributing to cell proliferation. UBE2M Protein, Human is the recombinant human-derived UBE2M protein, expressed by E. coli , with tag free. The total length of UBE2M Protein, Human is 183 a.a., with molecular weight of 19-25.0 kDa.
  • HY-P71409
    UBE2T Protein, Human (His)

    Ubiquitin-Conjugating Enzyme E2 T; Cell Proliferation-Inducing Gene 50 Protein; Ubiquitin Carrier Protein T; Ubiquitin-Protein Ligase T; UBE2T

    Human E. coli
    UBE2T Protein, an essential ubiquitination component, serves as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to proteins. This multifaceted enzyme catalyzes monoubiquitination, crucial in MMC-induced DNA repair processes. Its significance is highlighted by specific association with the Fanconi anemia complex, collaborating with FANCL for FANCD2 monoubiquitination in the DNA damage response pathway. Beyond DNA repair, UBE2T mediates monoubiquitination of FANCL and FANCI and potentially contributes to BRCA1 ubiquitination and degradation. In vitro, UBE2T promotes various polyubiquitination types, showcasing involvement in diverse ubiquitin-dependent processes. UBE2T Protein, Human (His) is the recombinant human-derived UBE2T protein, expressed by E. coli, with N-6*His labeled tag. The total length of UBE2T Protein, Human (His) is 197 a.a., with molecular weight of 25-30 kDa.
  • HY-P74484
    UBASH3B/STS1 Protein, Human (His)

    Ubiquitin-associated and SH3 domain-containing protein B; STS-1; TULA-2; UBASH3B

    Human E. coli
    UBASH3B/STS1 protein blocks CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases, leading to the accumulation of activating receptors such as T cell receptors and EGFR on the cell surface. It exhibits tyrosine phosphatase activity against substrates such as EGFR, FAK, SYK and ZAP70. UBASH3B/STS1 Protein, Human (His) is the recombinant human-derived UBASH3B/STS1 protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of UBASH3B/STS1 Protein, Human (His) is 268 a.a., with molecular weight of ~30 kDa.
  • HY-P75894
    ITCH/AIP4 Protein, Human (solution)

    E3 ubiquitin-protein ligase Itchy homolog; AIP4; NAPP1; ITCH

    Human E. coli
    The ITCH/AIP4 protein, a member of the Nedd4 family of ubiquitin ligases, transfers ubiquitin to protein substrates for lysosomal degradation. It regulates cell differentiation and immune responses and is associated with autoimmune disease. Alternative splicing produces multiple isoforms. ITCH/AIP4 is expressed ubiquitously, with notable levels in the testis, esophagus, and 25 other tissues. ITCH/AIP4 Protein, Human (solution) is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes).
  • HY-P76008
    RNF43 Protein, Human (HEK293, His)

    E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RNF43

    Human HEK293
    RNF43 protein is encoded by the RNF43 gene and is a RING-type E3 ubiquitin ligase. It negatively regulates Wnt signaling by increasing ubiquitination of Frizzled receptors, resulting in reduced surface levels of these receptors. RNF43 Protein, Human (HEK293, His) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-His labeled tag.
  • HY-P76687
    UBASH3A Protein, Human (His)

    Ubiquitin-associated and SH3 domain-containing protein A; CLIP4; STS-2; TULA-1

    Human E. coli
    UBASH3A interferes with CBL-mediated downregulation and degradation of receptor-type tyrosine kinases and promotes the accumulation of activating receptors such as T cell receptors, EGFR and PDGFRB on the cell surface. It exhibits minimal protein tyrosine phosphatase activity and may act as a dominant negative regulator of UBASH3B-dependent dephosphorylation. UBASH3A Protein, Human (His) is the recombinant human-derived UBASH3A protein, expressed by E. coli , with N-His labeled tag.
  • HY-P77271
    UBE2G1 Protein, Human

    Ubiquitin-conjugating enzyme E2 G1; E217K; UBC7; UBE2G1; UBE2G

    Human E. coli
    UBE2G1 Protein, a crucial ubiquitin-proteasome system player, acts as an E2 ubiquitin-conjugating enzyme, facilitating ubiquitin attachment to diverse protein substrates. In vitro, UBE2G1 demonstrates catalytic competence in 'Lys-48' and 'Lys-63'-linked polyubiquitination reactions. This multifaceted enzyme is implicated in potential roles, including the degradation of muscle-specific proteins, emphasizing its involvement in cellular processes related to protein turnover. Additionally, UBE2G1 mediates the polyubiquitination of CYP3A4, a cytochrome P450 enzyme, underscoring its significance in regulating specific cellular pathways. UBE2G1 Protein, Human is the recombinant human-derived UBE2G1 protein, expressed by E. coli, with tag free. The total length of UBE2G1 Protein, Human is 170 a.a., with molecular weight of ~19.5 kDa.
  • HY-P77272
    UBE2W Protein, Human (His)

    Ubiquitin-conjugating enzyme E2 W; UBC-16; Ubiquitin-protein ligase W; UBC16

    Human E. coli
    UBE2W protein is a multifunctional ubiquitin-conjugating enzyme that accepts ubiquitin in the E1 complex and catalyzes its covalent attachment to different substrates. Notably, UBE2W uniquely monoubiquitylates the N-terminus, including ATXN3, MAPT/TAU, POLR2H/RPB8, and STUB1/CHIP, showing specificity for disordered N-termini. UBE2W Protein, Human (His) is the recombinant human-derived UBE2W protein, expressed by E. coli , with N-His labeled tag.
Cat. No. Product Name Effect Purity