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  3. Adenovirus Packaging Services

Adenovirus Packaging Services

In Stock Adenovirus

Adenovirus (ADV) is a non-enveloped, linear, double-stranded DNA virus. It enters cells through receptor-mediated endocytosis, initiated by the binding of its fiber protein to cell surface receptors. The virus then escapes the endosome and translocates to the cytoplasm and nucleus, where it hijacks the host cell's transcription and translation machinery to initiate viral replication and assembly. A complete adenovirus life cycle culminates in cell lysis, releasing viral particles.

Adenovirus has the ability to infect a wide range of mammalian cells. As a gene delivery tool, it offers several advantages, including broad host range, high transduction efficiency, and strong expression activity. These features make adenovirus widely used for in vitro and certain in vivo gene delivery applications, and a valuable tool in gene therapy, vaccine development, and oncolytic virotherapy.

Figure 1. The life cycle of adenovirus.

Key Features of Adenovirus

• High Transduction Efficiency:

Adenovirus vectors exhibit near 100% transduction efficiency in vitro, making them the popular choice for hard-to-transfect cells.

• Large Transgene Capacity:

Capable of accommodating foreign genes up to 7 kb in size.

• Non-Integrating:

Adenovirus vectors does not integrate into the host genome, allowing direct translation and transcription avoiding the risk of insertional mutagenesis.

• Broad Tropism:

Capable of infecting both dividing and non-dividing cells, ensuring efficient gene delivery across a wide range of cell types.

• Hepatotropism:

Adenovirus vectors have a natural affinity for liver cells, making it particularly effective for liver-targeted applications in vivo. However, due to their high immunogenicity, adenovirus vectors may cause inflammation in certain animal models or tissues sensitive to external stimuli. When using adenovirus vectors for in vivo applications, be cautious to minimize potential inflammatory responses.

Adenovirus Packaging Services

We offer a comprehensive range of adenovirus products and services to support efficient manipulation of both coding and non-coding genes. For coding genes, we provide solutions for overexpression, knockdown (RNAi), and knockout (CRISPR-Cas9). For non-coding genes, we cover lncRNA (long non-coding RNA), microRNA, and circRNA (circular RNA). These products and services provide flexible and powerful tools for gene function research and disease mechanism exploration.

Our adenovirus production and quality control follow internationally recognized standards. We use AdEasy or AdMAX Adenovirus Packaging System to package adenoviral particles in HEK-293 cells. The viral particles are purified via ultracentrifugation, and the titer is tested by capsid protein immunoassay. The adenoviruses we deliver have titers exceeding 1E+10 PFU/mL and are free from mycoplasma, endotoxins, and other contaminants, ensuring suitability for various experimental applications.

Note: Sequences that negatively affect the adenovirus packaging process, such as toxic genes, genes that disrupt the integrity of packaging cells, sequences that disrupt the integrity of the adenoviral RNA genome, repetitive sequences, or sequences with high GC content, may not guarantee a titer >1E+10 PFU/mL.

Table 1.MedChemExpress Adenovirus Products and Packaging Service Types

Types Products Titers Application
Overexpression Adenovirus Adenovirus Regular Titer 1E+10PFU/mL
High Titer 1E+11PFU/mL		 In Vivo or In Vitro Applications
circRNA Overexpression Adenovirus
miRNA Overexpression Adenovirus
Cre-Expressing Adenovirus
Knockdown Adenovirus
Knockout Adenovirus (Cas9)
Service Workflow

Customers could provide information such as the target gene name, sequence, and experimental purpose.

Selection of Adenovirus Expression Vector
Preparation of Helper Plasmids

Co-transfection into 293T Cells Adenovirus Packaging and Purification Adenovirus Quality Testing

High-Quality Adenovirus
Project Report

Shipping and Storage

• Adenovirus products are shipped on dry ice.

• Upon receipt, store the virus at -80°C to maintain optimal activity.

• After 6 months of storage, we recommend re-measuring the titer before use to ensure consistent performance.

• Avoid repeated freeze-thaw cycles, as this could reduce the titer and compromise the integrity of the virus.

Service Consultation
Adenovirus packaging custom services require evaluation to determine the appropriate scheme and pricing. For additional information about service price and technical specifics, please send an email to sales@MedChemExpress.com or contact the sales staff of MedChemExpress directly.