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  3. Lentivirus Packaging Services

Lentivirus Packaging Services

In-stock Lentivirus

Lentivirus vectors, derived from the human immunodeficiency virus type 1 (HIV-1), are powerful tools for gene delivery in gene therapy and research. They exhibit broad tropism and high efficiency in infecting both dividing and non-dividing cells, and enable long-term stable expression of transgenes. These features make them a better choice for gene delivery applications.

Once the lentiviral genome enters the cell, it undergoes reverse transcription in the cytoplasm, forming a pre-integration complex. This complex then enters the nucleus, where the viral DNA integrates into the host cell's genome. The integrated DNA is then transcribed into mRNA or small RNA, the mRNA is transported back to the cytoplasm for translation and expression of the target protein. Lentivirus-mediated gene expression or small RNA knockdown is sustained and stable, and can be inherited by daughter cells during cell division.

Figure 1. Schematic diagram showing production of lentiviral particles and stable integration of the target DNA into the host genome.

Key Features of Lentivirus

• Stable Expression:

Lentivirus integrates the transgene into the host genome, ensuring long-term stable expression that persists through cell division. This feature makes lentivirus an ideal tool for generating stable cell lines.

• Broad Tropism:

Lentivirus efficiently infects both dividing and non-dividing cells, making it suitable for a wide range of cell types, including tumor cells, stem cells, cardiomyocytes, and neurons. This feature makes lentivirus a popular choice for in vitro cell experiments.

• Low Immunogenicity:

Lentivirus can be directly injected into animals without readily causing an immune response, which makes it suitable for in vivo studies.

• High Safety:

Lentivirus is based on self-inactivating, replication-deficient viral strains, ensuring safety in experimentation. For example, they are already used in CAR-T cell therapy.

Lentivirus Packaging Services

MedChemExpress offer a comprehensive range of lentivirus products and services to support solutions of both coding and non-coding genes. For coding genes, we provide solutions for overexpression, knockdown (RNAi), and knockout (CRISPR-Cas9). For non-coding genes, we cover lncRNA (long non-coding RNA), microRNA, and circRNA (circular RNA). These products and services provide flexible and powerful tools for gene function research and disease mechanism exploration.

Our lentivirus production and quality control follow internationally recognized standards. We use a three-plasmid or four-plasmid system to package lentiviral particles in 293T cells. The viral particles are purified via ultracentrifugation, and the titer is tested by qPCR. The lentiviruses we deliver have titers exceeding 1E+8TU/mL and are free from mycoplasma, endotoxins, and other contaminants, ensuring suitability for various experimental applications.

Note: Sequences that negatively affect the lentivirus packaging process, such as toxic genes, genes that disrupt the integrity of packaging cells, sequences that disrupt the integrity of the lentiviral RNA genome, repetitive sequences, or sequences with high GC content, may not guarantee a titer >1E+8TU/mL.

Table 1. MedChemExpress Lentivirus Products and Packaging Service Types

Products and Services Type Titers Application
Products
In Stock		 Overexpression Negative Control Regular Titer Lentivirus
OR
High Titer Lentivirus		 Regular Titer 1E+8TU/mL
High Titer 1E+9TU/mL		 Regular titer lentivirus is suitable for infecting common cell lines.
High titer lentivirus is suitable for difficult-to-transfect cell lines (e.g. suspension cells) or for in vivo studies.
Knockdown Negative Control
General Fluorescence Control
Dual-Labeled/Triple-Labeled Lentivirus
Autophagy Lentivirus
lncRNA Lentivirus
Custom
Service		 Overexpression Lentivirus
shRNA Knockdown Lentivirus (Single)
shRNA Knockdown Lentivirus (Full Set)
Knockout Lentivirus (Cas9)
Service Workflow

Customers could provide information such as the target gene name, sequence, and experimental purpose.

Selection of Lentiviral Expression Vector
Preparation of Helper Plasmids

Co-transfection into 293T Cells Lentivirus Packaging and Purification Lentivirus Quality Testing

High-Quality Lentivirus
Project Repor

Shipping and Storage

• Lentivirus products is shipped on dry ice.

• Upon receipt, store the virus at -80°C to maintain optimal activity.

• After 6 months of storage, we recommend re-measuring the titer before use to ensure consistent performance.

• Avoid repeated freeze-thaw cycles, as this could reduce the titer and compromise the integrity of the virus.

Service Consultation
Lentivirus packaging custom services require evaluation to determine the appropriate scheme and pricing. For additional information about service price and technical specifics, please send an email to sales@MedChemExpress.com or contact the sales staff of MedChemExpress directly.