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  3. Phosphatase Inhibitor Cocktail
  4. Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 

Cat. No.: HY-K0022
Manual COA SDS Technical Support

Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs).

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390 Publications Citing Use of MCE Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

WB

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Mar 14;56(3):620-634.e11.  [Abstract]

    After chronic SDS induction with or without idazoxan administration (1 mg/kg i.p.) for 7 days, mice were used for SIT testing (Ci), measuring DBH activity (Cii) and NE concentration (Ciii) in the LC, analyzing protein expression of LBP and GAPDH in the brain (Civ and Cv), determining protein expression of BDNF, SYN, and GAPDH in the hippocampus (Cvi), and performing Golgi-Cox staining of dendritic ridge density in the CA3 (Cvii). n = 6–8, and data are representative of at least two independent e

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322.  [Abstract]

    Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 Nov;21(11):1251-1265.  [Abstract]

    The cells were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
    Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2023 May;26(5):751-764.  [Abstract]

    Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322.  [Abstract]

      Customer Validation
      Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Immunity. 2023 Mar 14;56(3):620-634.e11.  [Abstract]

      Customer Validation
      After chronic SDS induction with or without idazoxan administration (1 mg/kg i.p.) for 7 days, mice were used for SIT testing (Ci), measuring DBH activity (Cii) and NE concentration (Ciii) in the LC, analyzing protein expression of LBP and GAPDH in the brain (Civ and Cv), determining protein expression of BDNF, SYN, and GAPDH in the hippocampus (Cvi), and performing Golgi-Cox staining of dendritic ridge density in the CA3 (Cvii). n = 6–8, and data are representative of at least two independent e

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Cell Mol Immunol. 2024 Aug 20.  [Abstract]

      Customer Validation
      The cells were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
      Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MCE. Usage Cited in: Nat Neurosci. 2023 Mar 27.  [Abstract]

      Customer Validation
      Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.

      • Description

      • Price Comparison

      • Storage

      • Protocol

      • Components

      • Documentation

      Description
      & Advantages

      Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.

      MCE Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs). It is used to maintain the phosphorylation status of proteins. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.
      Price Comparison
      Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH<sub>2</sub>O) Price Comparison
      Storage

      -20℃, 2 years
      Protocol

      Thaw at room temperature, add the inhibitor mixture to the lysate at 1:100 (v/v), and mix thoroughly.
      Components
      Cat. No. Product Name Package
      HY-K0022-1 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL
      HY-K0022-10 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 10
      HY-K0022-50 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 50
      HY-K0022-100 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 100
      Documentation

      Manual (1104 KB) SDS (4575 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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