Xylobiose (Synonyms: 1,4-β-D-Xylobiose; 1,4-D-Xylobiose)

Cat. No.: HY-N2468 Purity: ≥98.0%: Data Sheet
SDS

COA
Handling Instructions
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Xylobiose (1,4-β-D-Xylobiose; 1,4-D-Xylobiose) is an orally active Claudin 2/CLDN2 inhibitor and HSP27 inducer. Xylobiose works by regulating intestinal barrier function and glucose and lipid metabolism-related signaling pathways. Xylobiose inhibits CLDN2 expression to reduce intestinal permeability, induces HSP27 to enhance cell protection, and regulates the miR-122a/miR-33a axis to inhibit liver lipid synthesis and improve insulin resistance. Xylobiose can strengthen intestinal barrier integrity, reduce blood sugar and blood lipid levels, and reduce oxidative stress and inflammatory response. Xylobiose can be used in the study of type 2 diabetes and metabolic syndrome.

For research use only. We do not sell to patients.

Xylobiose Chemical Structure

CAS No. : 6860-47-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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HSP40 HSP70 HSP90 HSP105 HSF1 HSP HSPA5

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Claudin-2

HSP27

In Vitro

Xylobiose (5-20 mg/mL; 3-24 h) down-regulates the gene and protein expression of Claudin 2 in human intestinal Caco-2 cells, up-regulates the gene and protein expression of heat shock proteins HSP27 and HSP70, and enhances the transepithelial electrical resistance (TER) of the cell monolayer, which may strengthen the intestinal barrier function by regulating signaling pathways such as PI3K^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Human intestinal Caco-2 cells
Concentration:	5, 10, 20 mg/mL
Incubation Time:	3, 6, 12, 24 h
Result:	Downregulated CLDN2 expression, which is involved in paracellular permeability, and upregulated HSP27 and HSP70 expression, which are cytoprotective proteins. These changes indicated an improvement in the cell's functional state rather than a direct impact on cell viability.

In Vivo

Xylobiose (5% replacement of dietary sucrose; dietary intake; daily; 6 weeks) improves glucose tolerance in the db/db mouse diabetic model, reduces fasting blood glucose, HbA1c, insulin levels and HOMA-IR, reduces plasma total triglycerides, regulate liver miR-122a and miR-33a expression, inhibits liver lipid production-related gene expression, and reduces liver oxidative stress and inflammatory response^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:
Dosage:
Administration:
Result:	Animal Model: C57BL/KsJ-db/db male mice (17-24 g, 5-week-old), type 2 diabetes model induced by genetic factors^[2] - Dosage (solvent): 5% xylobiose replacing sucrose in the diet (dissolved in the diet) - Administration: Dietary intake, daily, for 6 weeks - Result: Did not affect the body weight change compared with the diabetic control group, while significantly reduced food and water intake.

Molecular Weight

282.24

Formula

C₁₀H₁₈O₉

CAS No.

6860-47-5

Appearance

Solid

Color

White to off-white

SMILES

O=C[C@H](O)[C@@H](O)[C@@H](CO)O[C@H]1[C@@H]([C@H]([C@H](O)CO1)O)O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 125 mg/mL (442.89 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5431 mL	17.7154 mL	35.4308 mL
5 mM	0.7086 mL	3.5431 mL	7.0862 mL
10 mM	0.3543 mL	1.7715 mL	3.5431 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (354.31 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (250 KB) HNMR (259 KB)

Handling Instructions (2659 KB)

References

[1]. Rini DM, et al. Xylobiose treatment strengthens intestinal barrier function by regulating claudin 2 and heat shock protein 27 expression in human Caco-2 cells. J Sci Food Agric. 2024 Mar 15;104(4):2518-2525. [Content Brief]

[2]. Lim E, et al. Xylobiose, an Alternative Sweetener, Ameliorates Diabetes-Related Metabolic Changes by Regulating Hepatic Lipogenesis and miR-122a/33a in db/db Mice. Nutrients. 2016 Dec 5;8(12):791 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.5431 mL	17.7154 mL	35.4308 mL	88.5771 mL
	5 mM	0.7086 mL	3.5431 mL	7.0862 mL	17.7154 mL
	10 mM	0.3543 mL	1.7715 mL	3.5431 mL	8.8577 mL
	15 mM	0.2362 mL	1.1810 mL	2.3621 mL	5.9051 mL
	20 mM	0.1772 mL	0.8858 mL	1.7715 mL	4.4289 mL
	25 mM	0.1417 mL	0.7086 mL	1.4172 mL	3.5431 mL
	30 mM	0.1181 mL	0.5905 mL	1.1810 mL	2.9526 mL
	40 mM	0.0886 mL	0.4429 mL	0.8858 mL	2.2144 mL
	50 mM	0.0709 mL	0.3543 mL	0.7086 mL	1.7715 mL
	60 mM	0.0591 mL	0.2953 mL	0.5905 mL	1.4763 mL
	80 mM	0.0443 mL	0.2214 mL	0.4429 mL	1.1072 mL
	100 mM	0.0354 mL	0.1772 mL	0.3543 mL	0.8858 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Xylobiose Related Classifications

Metabolic Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Xylobiose6860-47-51,4-β-D-Xylobiose 1,4-D-XylobioseTNF ReceptorClaudinHSPTumor Necrosis Factor ReceptorTNFRHeat shock proteinsDisaccharide1,4-D-XylobioseInhibitorinhibitorinhibit

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