2. MAPK/ERK Pathway PI3K/Akt/mTOR Epigenetics Anti-infection Immunology/Inflammation Metabolic Enzyme/Protease Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Apoptosis NF-κB
  3. p38 MAPK AMPK Bacterial Fungal Interleukin Related PPAR CDK PI3K Akt mTOR Apoptosis Reactive Oxygen Species
  4. Sinigrin hydrate

Sinigrin hydrate  (Synonyms: Allyl-glucosinolate hydrate; 2-Propenyl-glucosinolate hydrate)

Cat. No.: HY-N2423 Purity: 99.77%
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Sinigrin (Allyl-glucosinolate) hydrate is an orally active glucosinolate found in cruciferous plants. Sinigrin hydrate possesses multiple activities such as anti-cancer, antibacterial, antifungal, anti-inflammatory, antioxidant, and inhibition of fat synthesis. Sinigrin hydrate can be used in the research of tumors, inflammatory, and metabolic diseases.

For research use only. We do not sell to patients.

Sinigrin hydrate Chemical Structure

Sinigrin hydrate Chemical Structure

CAS No. : 64550-88-5

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Sinigrin hydrate:

Sinigrin hydrate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Sinigrin hydrate

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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PPARα PPARβ/δ PPARγ PPAR PPARδ

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Akt Akt1 Akt2 Akt3

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mTOR mTORC1 mTORC2

  • Biological Activity

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  • References

  • Customer Review

Description

Sinigrin (Allyl-glucosinolate) hydrate is an orally active glucosinolate found in cruciferous plants. Sinigrin hydrate possesses multiple activities such as anti-cancer, antibacterial, antifungal, anti-inflammatory, antioxidant, and inhibition of fat synthesis. Sinigrin hydrate can be used in the research of tumors, inflammatory, and metabolic diseases[1][2][3][4].

IC50 & Target[1][2]

PPARγ

 

IL-6

 

IL-1β

 

CDK4

 

CDK6

 

In Vitro

Sinigrin (1-100 μg/mL; 1-8 days) hydrate inhibits lipid accumulation, downregulates the expression of adipogenesis-related genes, suppresses the production of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, IL-18), and induces cell cycle arrest in 3T3-L1 cells. The mechanism involves activation of the AMPK pathway and inhibition of the MAPK pathway[1].
Sinigrin (5-30 μM; 24-48 h) hydrate induces cell cycle arrest in the S phase and promotes apoptosis, while triggering ROS-dependent oxidative stress damage by inhibiting PI3K/AKT/mTOR phosphorylation in MCF-7 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sinigrin hydrate Related Antibodies

Cell Cycle Analysis[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 100 μg/mL
Incubation Time: 24 h
Result: Arrested cells in the G0/G1phase of the cell cycle and increased the expression of p21 and p27.

Western Blot Analysis[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 1, 10, and 100 μg/mL
Incubation Time: 8 days
Result: Remarkably inhibited the accumulation of lipid droplets and adipogenesis by downregulating the expression of CCAAT-enhancer-binding protein α (C/EBPα), PPARγ, leptin and aP2.

RT-PCR[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 100 μg/mL
Incubation Time: 24 h
Result: Inhibited the production of pro-inflammatory cytokines including TNF-α and IL-6, IL-1β and IL-18.

Western Blot Analysis[2]

Cell Line: MCF-7 cells
Concentration: 20 μM
Incubation Time: 24 and 48 h
Result: Inhibited the levels of cyclin-D1, PCNA, CDK4, and CDK6.
In Vivo

Sinigrin (15-30 mg/kg; oral administration; 12 days) hydrate exerts anti-inflammatory and ameliorative effects in DSS (HY-116282C)-induced ulcerative colitis mouse models[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BALB/c mice (20-25 gm) treated DSS (HY-116282C)[3]
Dosage: 15 and 30 mg/kg
Administration: Oral administration; 12 days
Result: Significantly mitigated the DSS-induced body weight loss, attenuated the colon length shrinkage, and improved the disease index score.
Successfully abrogated the DSS-induced IL-17 levels and improved the colonic barrier in colon tissues.
Molecular Weight

415.48

Formula

C10H18KNO10S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@@H](O)[C@H](S/C(CC=C)=N/OS(=O)(O[K])=O)O[C@H](CO)[C@H]1O.O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 50 mg/mL (120.34 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4069 mL 12.0343 mL 24.0685 mL
5 mM 0.4814 mL 2.4069 mL 4.8137 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.4069 mL 12.0343 mL 24.0685 mL 60.1714 mL
5 mM 0.4814 mL 2.4069 mL 4.8137 mL 12.0343 mL
10 mM 0.2407 mL 1.2034 mL 2.4069 mL 6.0171 mL
15 mM 0.1605 mL 0.8023 mL 1.6046 mL 4.0114 mL
20 mM 0.1203 mL 0.6017 mL 1.2034 mL 3.0086 mL
25 mM 0.0963 mL 0.4814 mL 0.9627 mL 2.4069 mL
30 mM 0.0802 mL 0.4011 mL 0.8023 mL 2.0057 mL
40 mM 0.0602 mL 0.3009 mL 0.6017 mL 1.5043 mL
50 mM 0.0481 mL 0.2407 mL 0.4814 mL 1.2034 mL
60 mM 0.0401 mL 0.2006 mL 0.4011 mL 1.0029 mL
80 mM 0.0301 mL 0.1504 mL 0.3009 mL 0.7521 mL
100 mM 0.0241 mL 0.1203 mL 0.2407 mL 0.6017 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Sinigrin hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sinigrin64550-88-5Allyl-glucosinolate2-Propenyl-glucosinolatep38 MAPKAMPKBacterialFungalInterleukin RelatedPPARCDKPI3KAktmTORApoptosisReactive Oxygen SpeciesAMP-activated protein kinaseILPeroxisome proliferator-activated receptorsCyclin dependent kinasePhosphoinositide 3-kinasePKBProtein kinase BMammalian target of Rapamycinadipogenesisadipocytedifferentiationulcerative colitis model3T3-L1 cellsMCF-7 cellsInhibitorinhibitorinhibit

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