2. Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. Toll-like Receptor (TLR) PKD HSP Bcl-2 Family Interleukin Related
  4. Polyinosinic-polycytidylic acid

Polyinosinic-polycytidylic acid  (Synonyms: Poly(I:C))

Cat. No.: HY-107202 Assay: 99.40%
SDS
COA
Handling Instructions Technical Support

Polyinosinic-polycytidylic acid (Poly(I:C)) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid can directly trigger cancer cells to undergo apoptosis.

For research use only. We do not sell to patients.

Polyinosinic-polycytidylic acid Chemical Structure

Polyinosinic-polycytidylic acid Chemical Structure

CAS No. : 24939-03-5

Size Price Stock Quantity
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 33 publication(s) in Google Scholar

Other Forms of Polyinosinic-polycytidylic acid:

Polyinosinic-polycytidylic acid Related Antibodies

Top Publications Citing Use of Products

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2021 Aug 15;418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

    View All Toll-like Receptor (TLR) Isoform Specific Products:

    View All Isoforms
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    View All PKD Isoform Specific Products:

    View All Isoforms
    PKD1 PKD2 PKD3

    View All HSP Isoform Specific Products:

    View All Isoforms
    HSP40 HSP70 HSP90 HSP105 HSF1 HSP HSPA5

    View All Bcl-2 Family Isoform Specific Products:

    View All Isoforms
    Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

    View All Interleukin Related Isoform Specific Products:

    View All Isoforms
    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Polyinosinic-polycytidylic acid (Poly(I:C)) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid can directly trigger cancer cells to undergo apoptosis[1][2].

    IC50 & Target

    Bcl-2

     

    Bax

     

    IL-17A

     

    IL-13

     

    HSP70

     

    In Vitro

    Polyinosinic-polycytidylic acid (0.5-5 μg/mL, 3-24 h) induces a dose- and time-dependent increase in paracellular permeability of immortalized airway epithelial cells[4].
    Polyinosinic-polycytidylic acid (5 μg/mL, 24 h) does not have cytotoxicity to 16HBE14o- cells[4].
    Polyinosinic-polycytidylic acid (5 μg/mL, 6 h) induces disruption of epithelial apical junctional complexes (AJCs) and tight junctions (TJs) in 16HBE14o- cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Polyinosinic-polycytidylic acid Related Antibodies

    Cell Cytotoxicity Assay[4]

    Cell Line: 16HBE14o- cells
    Concentration: 5 μg/mL
    Incubation Time: 24 h
    Result: Did not lead to significant accumulation of LDH in cell-culture medium
    In Vivo

    Polyinosinic-polycytidylic acid (2.5-10 mg/mL, Stereotaxic injection, single dose) induces a sustained inflammatory reaction in the substantia nigra (SN) and in the dorsolateral striatum. [2].
    Polyinosinic-polycytidylic acid (10 μg/mouse, Intraperitoneal injection, single dose) decreases lung tumor growth in mice[3].
    Polyinosinic-polycytidylic acid (1.25 mg/kg, Intraperitoneal injection, single dose) exerts therapeutic effects against cerebral I/R injury through the downregulation of TLR4/MyD88 signaling via TLR3 in MCAO model mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: lung tumor-bearing mice[3]
    Dosage: 10 μg/mouse
    Administration: Intraperitoneal injection (i.p.)
    Result: Induced a significant decrease in the growth of pulmonary metastases in tumor-bearing mice.
    Reduced the amount of lung foci to ≈ 40%.
    Significantly increased BAL fluid cell numbers.
    Increased the level of INF-γ and IL-17A, decreased the levels of IL-13.
    Increased TLR3 expression.
    Animal Model: Middle cerebral artery occlusion (MCAO) model mice[5]
    Dosage: 1.25 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Reduced focal cerebral I/R injury.
    Increased the expression of Bcl2, Hsp27, and Hsp70, decreased Bax expression, and reduced cellular degeneration and apoptosis.
    Protected against cerebral ischemia and conferred protection against cerebral I/R injury through the downregulation of TLR4 signaling via TLR3.
    Formula

    (C10H13N4O8P)x.(C9H14N3O8P)x
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@@H](O[C@H](COP(O)(O)=O)[C@H]1O)N2C3=C(C(NC=N3)=O)N=C2.O[C@H]4[C@H](N5C(N=C(N)C=C5)=O)O[C@H](COP(O)(O)=O)[C@H]4O.[x].[x]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (Need ultrasonic)

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL; Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.40%

    SDS (393 KB)

    COA (305 KB)

    Handling Instructions (2659 KB)
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Polyinosinic-polycytidylic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Polyinosinic-polycytidylic acid24939-03-5Poly(I:C)Toll-like Receptor (TLR)PKDHSPBcl-2 FamilyInterleukin RelatedProtein kinase DHeat shock proteinsIL16HBE14o- cellsagonistlung tumor-bearing miceMiddle cerebral artery occlusion (MCAO) model miceInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Polyinosinic-polycytidylic acid
    Cat. No.:
    HY-107202
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.