2. Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. Toll-like Receptor (TLR) PKD HSP Bcl-2 Family Interleukin Related Apoptosis
  4. Polyinosinic-polycytidylic acid

Polyinosinic-polycytidylic acid  (Synonyms: Poly(I:C))

Cat. No.: HY-107202 Assay: 99.4%
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Polyinosinic-polycytidylic acid (Poly(I:C)) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid can directly trigger cancer cells to undergo apoptosis.

For research use only. We do not sell to patients.

CAS No. : 24939-03-5

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Customer Review

Based on 43 publication(s) in Google Scholar

Other Forms of Polyinosinic-polycytidylic acid:

Polyinosinic-polycytidylic acid Related Antibodies

Top Publications Citing Use of Products

38 Publications Citing Use of MCE Polyinosinic-polycytidylic acid

IP
RT-PCR
IF

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2021 Aug 15:418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

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    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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    Description

    Polyinosinic-polycytidylic acid (Poly(I:C)) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid can directly trigger cancer cells to undergo apoptosis[1][2].

    IC50 & Target

    Bcl-2

     

    Bax

     

    IL-17A

     

    IL-13

     

    HSP70

     

    In Vitro

    Polyinosinic-polycytidylic acid (0.5-5 μg/mL, 3-24 h) induces a dose- and time-dependent increase in paracellular permeability of immortalized airway epithelial cells[4].
    Polyinosinic-polycytidylic acid (5 μg/mL, 24 h) does not have cytotoxicity to 16HBE14o- cells[4].
    Polyinosinic-polycytidylic acid (5 μg/mL, 6 h) induces disruption of epithelial apical junctional complexes (AJCs) and tight junctions (TJs) in 16HBE14o- cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Polyinosinic-polycytidylic acid Related Antibodies

    Cell Cytotoxicity Assay[4]

    Cell Line: 16HBE14o- cells
    Concentration: 5 μg/mL
    Incubation Time: 24 h
    Result: Did not lead to significant accumulation of LDH in cell-culture medium.
    In Vivo

    Polyinosinic-polycytidylic acid (2.5-10 mg/mL, Stereotaxic injection, single dose) induces a sustained inflammatory reaction in the substantia nigra (SN) and in the dorsolateral striatum[2].
    Polyinosinic-polycytidylic acid (10 μg/mouse, Intraperitoneal injection, single dose) decreases lung tumor growth in mice[3].
    Polyinosinic-polycytidylic acid (1.25 mg/kg, Intraperitoneal injection, single dose) exerts therapeutic effects against cerebral I/R injury through the downregulation of TLR4/MyD88 signaling via TLR3 in MCAO model mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: lung tumor-bearing mice[3]
    Dosage: 10 μg/mouse
    Administration: Intraperitoneal injection (i.p.)
    Result: Induced a significant decrease in the growth of pulmonary metastases in tumor-bearing mice.
    Reduced the amount of lung foci to ≈ 40%.
    Significantly increased BAL fluid cell numbers.
    Increased the level of INF-γ and IL-17A, decreased the levels of IL-13.
    Increased TLR3 expression.
    Animal Model: Middle cerebral artery occlusion (MCAO) model mice[5]
    Dosage: 1.25 mg/kg
    Administration: Intraperitoneal injection (i.p.)
    Result: Reduced focal cerebral I/R injury.
    Increased the expression of Bcl2, Hsp27, and Hsp70, decreased Bax expression, and reduced cellular degeneration and apoptosis.
    Protected against cerebral ischemia and conferred protection against cerebral I/R injury through the downregulation of TLR4 signaling via TLR3.
    Formula

    (C10H13N4O8P)x.(C9H14N3O8P)x
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@@H](O[C@H](COP(O)(O)=O)[C@H]1O)N2C3=C(C(NC=N3)=O)N=C2.O[C@H]4[C@H](N5C(N=C(N)C=C5)=O)O[C@H](COP(O)(O)=O)[C@H]4O.[x].[x]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (Need ultrasonic)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL; Clear solution; Need ultrasonic

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    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.4%

    SDS (393 KB)

    COA (245 KB)

    Handling Instructions (2659 KB)
    References
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    Polyinosinic-polycytidylic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Polyinosinic-polycytidylic acid24939-03-5Poly(I:C)Toll-like Receptor (TLR)PKDHSPBcl-2 FamilyInterleukin RelatedApoptosisProtein kinase DHeat shock proteinsIL16HBE14o- cellsagonistlung tumor-bearing miceMiddle cerebral artery occlusion (MCAO) model miceInhibitorinhibitorinhibit

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