2. Immunology/Inflammation Apoptosis
  3. Toll-like Receptor (TLR) Apoptosis
  4. Poly (I:C):Kanamycin (1:1) (sodium)

Poly (I:C):Kanamycin (1:1) sodium is an isometric complex of Poly (I:C) (HY-135748) and Kanamycin (HY-16566). Poly(I:C) sodium, a synthetic analog of double-stranded RNA, is a TLR3 and retinoic acid-inducible gene I receptor (RIG-I and b>MDA5) agonist. Poly(I:C) sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses and induce apoptosis in cancer cells. Kanamycin is an orally active antibacterial agent (Gram-negative/positive bacteria) that inhibits translocation and causes miscoding by binding to the 70S ribosomal subunit. Kanamycin shows good inhibitory activity against Mycobacterium tuberculosis (susceptible and drug-resistant) and Klebsiella pneumoniae, and can be used in the research of tuberculosis and pneumonia.

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Poly (I:C):Kanamycin (1:1) (sodium) Chemical Structure

Poly (I:C):Kanamycin (1:1) (sodium) Chemical Structure

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Based on 33 publication(s) in Google Scholar

Other Forms of Poly (I:C):Kanamycin (1:1) (sodium):

Poly (I:C):Kanamycin (1:1) (sodium) Related Antibodies

Top Publications Citing Use of Products

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Poly (I:C):Kanamycin (1:1) (sodium) purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2021 Aug 15;418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

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    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

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    Description

    Poly (I:C):Kanamycin (1:1) sodium is an isometric complex of Poly (I:C) (HY-135748) and Kanamycin (HY-16566). Poly(I:C) sodium, a synthetic analog of double-stranded RNA, is a TLR3 and retinoic acid-inducible gene I receptor (RIG-I and b>MDA5) agonist. Poly(I:C) sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses and induce apoptosis in cancer cells[1][2]. Kanamycin is an orally active antibacterial agent (Gram-negative/positive bacteria) that inhibits translocation and causes miscoding by binding to the 70S ribosomal subunit. Kanamycin shows good inhibitory activity against Mycobacterium tuberculosis (susceptible and drug-resistant) and Klebsiella pneumoniae, and can be used in the research of tuberculosis and pneumonia[3][4][5][6].

    IC50 & Target

    TLR3[2], RIG-I[2], MDA5[2], apoptosis[2]
    In Vitro

    Poly (I:C) sodium (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours[1].
    Poly (I:C) sodium (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells[1].
    Poly (I:C) sodium (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Poly (I:C) sodium required RIG-I and MDA-5, respectively, and that required IPS-1[1].
    Poly (I:C) sodiumd (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells[1].

    Kanamycin (0.1-100 μg/mL; 2 weeks) exhibits good antibacterial activity (MIC=1-5 μg/mL) to various strains of mycobacteria in vitro[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Poly (I:C):Kanamycin (1:1) (sodium) Related Antibodies
    In Vivo

    Poly (I:C) sodium treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Poly (I:C) sodium[1].

    Kanamycin (2, 4 mg/kg; s.c.; once daily, 6 times a week for 3 weeks) inhibits growth of bovine tubercle bacilli in lung and spleen of mice[3].
    Kanamycin (1.25, 5 mg/kg; s.c.; single (at 3 h after infection)) inhibits the multiplication of K. pneumonia DT-S in lung, trachea, and blood of mice and in proportion to the dose administration, and also increases the survival rate of mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [Poly (I:C):Kanamycin (1:1) (sodium)]
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    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

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    Poly (I:C):Kanamycin (1:1) (sodium) Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Poly (I:C):Kanamycin (1:1) (sodium)Toll-like Receptor (TLR)ApoptosisTLR3RIG-IMDA5immunotherapytumormicroenvironment apoptosisdsRNAproperannealingdouble-strandedRNAInhibitorinhibitorinhibit

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