2. Fluorescent Dye
  3. Cell Behavior analysis Probes

Cell Behavior analysis Probes

Cells exhibit dynamic behaviors during proliferation, migration, differentiation and apoptosis, directly influencing tissue development, immune responses and disease progression. Cell function analysis dyes enable real-time monitoring of the dynamics of key structures or molecules, providing a convenient and intuitive tool for cellular proliferation investigation and active species detection.The fluorescent stock solutions are easy to use with minimized reagent loss, while ensuring experimental reproducibility.

Cell Behavior analysis Probes (21):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-DY1018
    FITC-Dextran (MW 4000) (solution) 60842-46-8
    FITC-Dextran (MW 4000) (solution) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 4000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 4000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.
    Solution Concentration: 2 mM
    FITC-Dextran (MW 4000) (solution)
  • HY-DY1002
    H2DCFDA (solution)
    H2DCFDA (DCFH-DA) (solution) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm).
    Solution Concentration: 10 mM.
    H2DCFDA (solution)
  • HY-DY1048
    DAF-FM DA (solution) 254109-22-3
    DAF-FM DA (Diaminofluorescein-FM diacetate) (solution) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).
    Solution Concentration: 2 mM
    DAF-FM DA (solution)
  • HY-DY1011
    PKH 26 (solution) 154214-55-8
    PKH 26 (solution) is a red fluorescent dye, PKH 26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm), which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell tracing studies.
    Solution Concentration: 5 mM
    PKH 26 (solution)
  • HY-DY1052
    Monochlorobimane (solution) 76421-73-3
    Monochlorobimane (Chlorobimane) (solution) is a fluorescent dye (λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays.
    Solution Concentration: 10 mM
    Monochlorobimane (solution)
  • HY-DY1041
    CellTracker Blue CMAC (solution) 147963-22-2
    CellTracker Blue CMAC (solution) is a non-fluorescent cell membrane permeable dye. The chloromethyl groups of CellTracker Blue CMAC are enzymatically cleaved by intracellular glutathione (GSH) to generate a fluorescent product (blue fluorescence, Ex/Em: 360/460 nm). CellTracker Blue CMAC is suitable for long-term cell tracking (up to 72 hours) and cell proliferation studies, and can also quantify GSH levels.
    Solution Concentration: 10 mM
    CellTracker Blue CMAC (solution)
  • HY-DY1015
    Dihydroethidium (solution) 104821-25-2
    Dihydroethidium (solution), also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation.
    Solution Concentration: 5 mM
    Dihydroethidium (solution)
  • HY-DY1059
    FM1-43 (solution) 149838-22-2
    FM1-43 (solution) is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.
    Solution Concentration: 10 mM
    FM1-43 (solution)
  • HY-DY1060
    Suc-Leu-Leu-Val-Tyr-AMC (solution) 94367-21-2
    Suc-Leu-Leu-Val-Tyr-AMC (solution) is a membrane-permeable calpain-specific fluorogenic substrate (Ex/Em = 390/480 nm).
    Solution Concentration: 20 mM
    Suc-Leu-Leu-Val-Tyr-AMC (solution)
  • HY-DY1019
    2-NBDG (solution) 186689-07-6
    2-NBDG (solution) is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.
    Solution Concentration: 5 mM
    2-NBDG (solution)
  • HY-DY1034
    4-MUNANA (solution) 76204-02-9
    4-MUNANA (solution) is a substrate of influenza virus neuraminidase (NA) with high selectivity and irreversible reaction. In the enzymatic reaction, 4-MUNANA is hydrolyzed by NA to generate fluorescent 4-methylumbelliferone (4-MU). By detecting the fluorescence intensity of 4-MU, quantitative analysis of NA activity can be achieved. 4-MUNANA can be used in influenza-related research, such as screening NA inhibitors, developing new anti-influenza drugs, and studying the infection mechanism of influenza viruses.
    Solution Concentration: 10 mM
    4-MUNANA (solution)
  • HY-DY1037
    Dihydrorhodamine 123 (solution) 109244-58-8
    Dihydrorhodamine 123 (DHR 123) (solution) is a non-fluorescent reactive oxygen species (ROS) indicator. Dihydrorhodamine 123 is oxidized to fluorescent Rhodamine 123 (HY-D0816) within cells in the presence of reactive oxygen species and it localizes in mitochondria (Ex/Em = 515/536 nm).
    Solution Concentration: 10 mM
    Dihydrorhodamine 123 (solution)
  • HY-DY1046
    Green CMFDA (solution) 136832-63-8
    Green CMFDA (solution) is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Solution Concentration: 10 mM
    Green CMFDA (solution)
  • HY-DY1044
    HADA hydrochloride (solution) 2253733-10-5
    HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λem~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation.
    Solution Concentration: 50 mM
    HADA hydrochloride (solution)
  • HY-DY1051
    Resazurin sodium (solution) 62758-13-8
    Resazurin sodium (Diazoresorcinol sodium) (solution) is a non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin sodium is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin sodium can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal).
    Solution Concentration: 10 mM
    Resazurin sodium (solution)
  • HY-DY1053
    1,3-Diphenylisobenzofuran (solution) 5471-63-6
    1,3-Diphenylisobenzofuran (DPBF) (solution) has been developed as a selective probe for the detection and quantitative determination of hydrogen peroxide in samples containing different reactive nitrogen and oxygen species (RNOS). DPBF is a fluorescent probe which, for almost 20 years, was believed to react in a highly specific manner toward some reactive oxygen species such as singlet oxygen and hydroxy, alkyloxy or alkylperoxy radicals.
    Solution Concentration: 10 mM
    1,3-Diphenylisobenzofuran (solution)
  • HY-DY1058
    2′,7′-Dichlorofluorescein diacetate (solution) 2044-85-1
    2′,7′-Dichlorofluorescein diacetate (DCFH2-DA) (solution) is a cell-permeable fluorescent probe. 2′,7′-Dichlorofluorescein diacetate can be used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon.
    Solution Concentration: 10 mM
    2′,7′-Dichlorofluorescein diacetate (solution)
  • HY-DY1033
    C12FDG (solution) 138777-25-0
    C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) (solution) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm).
    Solution Concentration: 10 mM
    C12FDG (solution)
  • HY-DY1016
    FITC-Dextran (MW 10000) (solution) 60842-46-8
    FITC-Dextran (MW 10000) (solution) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.
    Solution Concentration: 2 mM
    FITC-Dextran (MW 10000) (solution)
  • HY-DY1036
    Calcein-AM (solution) 148504-34-1
    Calcein AM (solution), has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein), which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm.
    Solution Concentration: 2 mM
    Calcein-AM (solution)