HADA hydrochloride (solution) 

HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λ_em~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation^[1].
Solution Concentration: 50 mM

1. Solution preparation^[2]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 250 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 250 μM with cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. S. aureus strains staining
2.1 Inoculate the S. aureus strain in a culture dish.
2.2 Add HADA hydrochloride (250 μM) to the cells.
2.3 Incubate cells at room temperature or 37°C for 30 min (optimized according to the experiment).
2.4 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

328.71

C₁₃H₁₃ClN₂O₆

2253733-10-5

N[C@H](CNC(C1=CC2=CC=C(O)C=C2OC1=O)=O)C(O)=O.[H]Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Erkin Kuru, et al. Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat Protoc. 2015 Jan;10(1):33-52.  [Content Brief]

  [2]. Xu Q, et al. Phage protein Gp11 blocks Staphylococcus aureus cell division by inhibiting peptidoglycan biosynthesis. mBio. 2024 Jun 12;15(6):e0067924.  [Content Brief]