Monochlorobimane (solution) 

Monochlorobimane (Chlorobimane) (solution) is a fluorescent dye (λ_ex=380 nm, λ_em=470 nm) to measure glutathione (GSH) in cellular assays^[1].
Solution Concentration: 10 mM

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solution preparation^[3]
1.1 Stock solution
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute with PBS or cell culture medium (optimized according to the experiment). The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are plated in clear-bottomed black 96-well microplates.
2.2 Add Monochlorobimane (1-10 μM) and incubate at 37°C for 30 minutes (optimized according to the experiment).
2.3 The fluorescence intensity is measured with fluorescence microscopy or Fluorescence microplate reader (Ex/Em = 380/480 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

226.66

C₁₀H₁₁ClN₂O₂

76421-73-3

O=C1N(C(C(C)=C2C)=O)N2C(CCl)=C1C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7.  [Content Brief]

  [2]. Manuela D. Machado, et al. Assessment of cellular reduced glutathione content in Pseudokirchneriella subcapitata using monochlorobimane. Journal of Applied Phycology volume 24, pages1509–1516(2012).

  [3]. Fotopoulou T, et al. Design and Synthesis of Novel Antioxidant 2-Substituted-5,7,8-Trimethyl-1,4-Benzoxazine Hybrids: Effects on Young and Senescent Fibroblasts. Antioxidants (Basel). 2024 Jun 29;13(7):798.  [Content Brief]