CellTracker Blue CMAC (solution) 

CellTracker Blue CMAC (solution) is a non-fluorescent cell membrane permeable dye. The chloromethyl groups of CellTracker Blue CMAC are enzymatically cleaved by intracellular glutathione (GSH) to generate a fluorescent product (blue fluorescence, Ex/Em: 360/460 nm). CellTracker Blue CMAC is suitable for long-term cell tracking (up to 72 hours) and cell proliferation studies, and can also quantify GSH levels^[1][2][3].
Solution Concentration: 10 mM

Procedure^[2][3]
1. Dye preparation
Stock solution: protect from light and store in aliquots.
Working solution: Dilute the stock solution with serum-free medium. Preheat the staining working solution to 37 ℃. The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
2. Cell labeling
Remove the cell culture medium and wash the cells with PBS.
Add the dye working solution and incubate at 37 ℃ for 30 minutes.
Remove the dye and wash 3 times with pre-warmed culture medium.
Change to fresh culture medium and continue culturing or experiment (fluorescence signal reaches peak value within 1-2 hours).
3. Detection
Fluorescence microscopy: (Ex 360 nm, Em 460 nm).
4. Precautions
4.1 Please adjust the CellTracker Blue CMAC working solution concentration, incubation temperature and time according to actual conditions.
4.2 This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, nor for food or medicine.
4.3 For your safety and health, please wear a lab coat and disposable gloves when operating.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

209.63

C₁₀H₈ClNO₂

147963-22-2

O=C1OC2=C(C(CCl)=C1)C=CC(N)=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. King N, et al. A new method of quantifying glutathione levels in freshly isolated single superfused rat cardiomyocytes. J Pharmacol Toxicol Methods. 2004 Nov-Dec;50(3):215-22.  [Content Brief]

  [2]. Dludla PV, et al. A dose-dependent effect of dimethyl sulfoxide on lipid content, cell viability and oxidative stress in 3T3-L1 adipocytes. Toxicol Rep. 2018 Oct 4;5:1014-1020.  [Content Brief]

  [3]. Horobin R W, et al. Reactive dyes for living cells: Applications, artefacts, and some comparisons with textile dyeing. Coloration technology, 2022,138(1):3-15.