Search Result
Results for "
fluorescence quenching
" in MedChemExpress (MCE) Product Catalog:
3
Biochemical Assay Reagents
| Cat. No. |
Product Name |
Target |
Research Areas |
Chemical Structure |
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- HY-D2864
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Fluorescent Dye
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Others
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Aza-CyBz is a selective NaCl-responsive fluorescent probe. Aza-CyBz visualizes plant salt stress by forming ordered aggregates with NaCl, leading to near-infrared fluorescence quenching. Aza-CyBz acts via electrostatic interaction between the cationic cyanine dye and NaCl to form J-aggregates, causing fluorescence quenching with a detection limit of 170 μM .
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- HY-D1030
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Fluorescent Dye
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Inflammation/Immunology
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Fluorescein Biotin is used as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or absorbance; the the fluorescence or absorbance of Fluorescein Biotin is quenched, upon binding to avidin or streptavidin.
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- HY-W012642
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DNA Stain
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Others
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2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. When 2-Aminopurine is inserted in anoligonucleotide, its fluorescence is highly quenched by stacking with the natural bases. 2-Aminopurine has been used to probe nucleic acid structure and dynamics .
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- HY-D1663
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Fluorescent Dye
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Others
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APTAB is a fluorescent cationic membrane probe. APTAB locates the anthracene-labeled molecules incorporated into model membranes by fluorescence quenching .
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- HY-D2924
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Fluorescent Dye
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Others
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CBG-549-QSY7 is a Dylight 549-labeled SNAP tag fluorescent probe (Ex/Em: 546 nm/580 nm), specially designed for non-washable fluorescence imaging applications. CBG-549-QSY7 employs an intramolecular FRET quenching mechanism: intramolecular fluorescence quenching occurs before binding to the SNAP tag, and upon binding, the quenching group is cleaved, releasing fluorescence. The background fluorescence of CBG-549-QSY7 is extremely low, and clear cell membrane images can be obtained after 5 minutes of incubation .
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- HY-173308
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Fluorescent Dye
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Others
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QSY-21 is a fluorescence quencher. QSY-21 possesses broad absorption in far red and NIR range, and can quench fluorescence of dyes that emit in this region. This is a carboxylic acid derivative. QSY-21 has intense absorption maximum at 661 nm, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common quencher for Cyanine5, Cyanine5.5, AF 647, or other spectrally similar fluorescent dyes.
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- HY-W012642A
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DNA Stain
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Others
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2-Aminopurine dihydrochloride is a fluorescent analog of guanosine. 2-Aminopurine dihydrochloride can be used as a fluorescence probe for nucleic acid structure and dynamics. Incorporating 2-Aminopurine dihydrochloride into DNA quenches its fluorescence .
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- HY-170466
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1-Palmitoyl-2-stearoyl-(12-doxyl)-sn-glycero-3-phosphocholine
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Biochemical Assay Reagents
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Others
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16:0-12 Doxyl PC (1-Palmitoyl-2-stearoyl-(12-doxyl)-sn-glycero-3-phosphocholine) is a fluorescence quencher, and its nitrogen oxide is located downstream of the sn-2 chain. 16:0-12 Doxyl PC has a better fluorescence quenching effect onthe fluorophore is buried within the hydrocarbon interior of the bilayer .
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- HY-D2579
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Biochemical Assay Reagents
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Others
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Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .
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- HY-126965
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Biochemical Assay Reagents
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Others
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Lipid Y is a potent monosaccharide which contains two bromines per chain. lipid Y binds to a tryptophan residue in a membrane protein and then quenches the fluorescence of the tryptophan residue .
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- HY-P4920
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Fluorescent Dye
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Others
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Mca-SEVNLDAEFK(Dnp)-NH2 contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence, such as can be used to measure the activity of BACE-1 .
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- HY-118213
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Fluorescent Dye
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Others
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N-(2-Hydroxyethyl)-1,8-naphthalimide is a fluorescent probe for detecting nucleic acids and their precursors. The fluorescence of N-(2-Hydroxyethyl)-1,8-naphthalimide will be quenched by these molecules.
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- HY-163204
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P-glycoprotein
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Cancer
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RhQ-DMB is a potent inhibitor of P-gp. RhQ-DMB exhibits high affinity and inhibitory activity in yeast strains expressing CmABCB1 .
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- HY-W007671R
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Endogenous Metabolite
Tyrosinase
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Others
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H-Tyr-OMe is an endogenous metabolite. H-Tyr-OMe quenches the fluorescence of carbon dots by oxidizing the phenolic hydroxyl group to quinone under the catalysis of Tyrosinase, and its amino and thiol binding ability can respond to changes in the concentration of biothiols .
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- HY-W007671
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Endogenous Metabolite
Tyrosinase
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Others
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H-Tyr-OMe is an endogenous metabolite. H-Tyr-OMe quenches the fluorescence of carbon dots by oxidizing the phenolic hydroxyl group to quinone under the catalysis of Tyrosinase, and its amino and thiol binding ability can respond to changes in the concentration of biothiols .
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- HY-42984
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Biochemical Assay Reagents
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Others
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BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
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- HY-D0720
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NSC-151912; L-6868
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Fluorescent Dye
Reactive Oxygen Species (ROS)
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Others
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Lucigenin is a chemiluminescence probe. Lucigenin can be used to detect the production of endogenous superoxide anion radical (O 2-). Lucigenin is extremely sensitive to chloride ions, while it combined with chloride ions, the fluorescence will be quenched. Lucigenin also can be used as a chloride indicator. Ex/Em=455/505 nm .
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- HY-P4919
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Beta-secretase
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Others
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Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
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- HY-W127703
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Fluorescent Dye
Biochemical Assay Reagents
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Others
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Octadecyl Rhodamine B chloride is an amphiphilic fluorescent probe with both membrane-binding ability and fluorescent properties, which easily forms aggregates in aqueous phase leading to self-quenching. Octadecyl Rhodamine B chloride can be used for cell membrane staining and further applied in membrane biology research. The maximum absorption/emission wavelengths of Octadecyl Rhodamine B chloride monomer in Triton X-100 are 565 nm/585 nm; the absorption peaks of aggregates in water are blue-shifted to 524 nm and 574 nm, with significantly quenched fluorescence .
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- HY-W013195
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Nucleoside Antimetabolite/Analog
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Cancer
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8-Bromoadenosine is an adenosine analog. Adenosine analogs mostly act as smooth muscle vasodilators and have also been shown to inhibit cancer progression. 8-Bromoadenosine can quench the intrinsic fluorescence of human serum albumin (HSA) through static quenching procedure. 8-Bromoadenosine can be used to synthesize adenosine phosphate, such as Acadesine (HY-13417), Clofarabine (HY-A0005), Fludarabine phosphate (HY-B0028) and Vidarabine (HY-B0277) .
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- HY-D1590
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Fluorescent Dye
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Others
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ODIPY Green 8-P2M is a novel thiol-reactive fluorescence probe based on the BODIPY fluorophore, the fluorescence is strongly quenched by d-PeT and then can be restored after reaction with thiol, resulting in an extremely high signal-to-noise ratio. ODIPY Green 8-P2M can be useful for detecting extremely low concentrations of protein in the gel after SDS-PAGE .
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- HY-160062
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Mucin
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Cancer
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S2.2 aptamer sodium is a nucleic acid aptamer targeting the mucin MUC1 and can be used for targeted imaging of MCF-7 cancer cells. S2.2 aptamer sodium was labeled with Cy5, and when fluorescent silicon nanodots (SiND) were present, the fluorescence was quenched; when MUC1 was also present, the fluorescence was restored. S2.2 aptamer sodium detects MUC1 with a linear range of 3.33-250 nM .
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- HY-D2761
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Fluorescent Dye
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Others
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DY-680-NHS ester is an amine reactive hydrophilic fluorochrome. It can be conjugated to an antibody with higher D/P ratio without causing fluorescence quenching and conjugate precipitation, and it is widely used in applications including western blotting, microscopy, flow cytometry, and cell-based assays. The spectrum has an excitation of 690nm and emission at 709nm.
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- HY-D0929
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α-Naphthol Orange
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Fluorescent Dye
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Others
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Orange I (α-Naphthol Orange) is an azo dye that can form a complex with Methylene blue (HY-14536) and cause fluorescence quenching. The equilibrium constant of the complex of Orange I and Methylene blue is 79900 mol -1·dm 3. Orange I can change the absorption spectrum of itself and Methylene blue .
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- HY-D1682
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Fluorescent Dye
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Others
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NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .
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- HY-175163
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Photosensitizer
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Cancer
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TPAPyN is a nitroreductase(NTR)-responsive type I photosensitizer. TPAPyN facilitates the imaging of hypoxic cancer cells and image-guided photodynamic therapy (PDT). TPAPyN does not emit fluorescence in the aqueous environment, but restores when NTR cleaves the nitrofuran quencher with aggregation-induced emission. TPAPyN can be used as a fluorescent probe for specific imaging of hypoxic cancer .
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- HY-P10922
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Cathepsin
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Others
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Cathepsin E substrate e is a substrate of Cathepsin E. Cathepsin E substrate e was designed in such a way that due to the close proximity of a Mca-donor and a Dnp-acceptor, a near complete intramolecular quenching effect was achieved in its intact state. After the proteolytic cleavage of the hydrophobic motif of the peptide substrate, both Mca and Dnp would be further apart, resulting in bright fluorescence .
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- HY-D1450
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S01448
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Fluorescent Dye
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Cancer
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IR-1048 is a nitroreductase (NTR)-responsive near-infrared fluorescence (NIR)/photoacoustic (PA) imaging probe and photothermal agent. IR-1048 is coupled with a nitroimidazole group to form IR-1048-MZ. IR-1048-MZ is catalytically reduced by NTR in a hypoxic environment, restoring strong near-infrared absorption and fluorescence emission (NIR II window), while activating the photothermal effect. IR-1048 relies on NTR-mediated electron transfer to relieve intramolecular fluorescence quenching, achieving specific imaging and photothermal ablation of tumor hypoxic areas. IR-1048 is mainly used for high-contrast NIR II/photoacoustic imaging and photothermal therapy research and tumor diagnosis of the tumor hypoxic microenvironment .
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- HY-P3123
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Fluorescent Dye
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Others
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Dnp-RPLALWRS is a fluorescent peptide substrate designed for human matrilysin (MMP-7). After enzymatic cleavage of Dnp-RPLALWRS at the alanine-leucine bond, the release of the Dnp group alleviates fluorescence quenching, thereby enabling real-time quantitative analysis of MMP-7 activity by increasing tryptophan emission. Dnp-RPLALWRS provides a sensitive and efficient detection method for kinetic studies and inhibitor screenin .
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- HY-P3749
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Fluorescent Dye
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Others
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Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage .
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- HY-P3123A
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Fluorescent Dye
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Others
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Dnp-RPLALWRS TFA is a fluorescent peptide substrate designed for human matrilysin (MMP-7). After enzymatic cleavage of Dnp-RPLALWRS TFA at the alanine-leucine bond, the release of the Dnp group alleviates fluorescence quenching, thereby enabling real-time quantitative analysis of MMP-7 activity by increasing tryptophan emission. Dnp-RPLALWRS TFA provides a sensitive and efficient detection method for kinetic studies and inhibitor screenin .
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- HY-P11302
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CHIKV
Virus Protease
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Infection
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DABCYL-RAGGYIFS-(E-EDANS)-NH2, an octapeptide, is a FRET-based substrate peptide of CHIKV nsP2protease with highly sensitivity. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can be cleaved by the active CHIKV nsP2protease, separating fluorophore and quencher and resulting in an increase of fluorescence signal. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can used for the determination of CHIKV nsP2protease activity .
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- HY-D2365
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Fluorescent Dye
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Others
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QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in
the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules .
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- HY-D2735
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Fluorescent Dye
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Others
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QSY-9-succinimidyl ester is a nonfluorescent acceptor molecule with a terminal NHS ester group. QSY-9-succinimidyl ester has a wide and intense quenching range from 500-600 nm that makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 500 nm to 600 nm. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
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- HY-W142692
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Biochemical Assay Reagents
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Others
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Dodecyl β-D-glucopyranoside is a non-ionic detergent and surfactant commonly used to solubilize and purify membrane proteins in biochemical research. Dodecyl β-D-glucopyranoside also interacts with bovine serum albumin (BSA) to quench its intrinsic fluorescence. The critical micelle concentration (CMC) of Dodecyl β-D-glucopyranoside (DG) and DG/BSA complex is 2.0 mM and 2.34 mM, respectively. Micelles can be formed in aqueous solutions above this concentration .
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- HY-P5485
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Bacterial
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Others
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Dabcyl-QALPETGEE-Edans is a synthetic peptide substrate for fluorescence resonance energy transfer (FRET) detection of Sortase A (SrtA) enzymatic activity. Dabcyl-QALPETGEE-Edans is labeled at both ends with the fluorescent group Edans (donor) and the quencher Dabcyl (acceptor), respectively. Dabcyl-QALPETGEE-Edans's central sequence contains a conserved motif specifically recognized by SrtA. Dabcyl-QALPETGEE-Edans demonstrates high sensitivity in vitro assays, enabling precise determination of the half-maximal inhibitory concentration of SrtA .
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- HY-D2204
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Phosphatase
SHP1
Fluorescent Dye
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Cancer
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SHP1-IN-1 (compound 5p) is a fluorescent probe for the protein tyrosine phosphatase SHP1 containing the Src homology 2 domain. SHP1-IN-1 has SHP1 inhibitory activity, selectivity for Fe 3+ ions and good fluorescence properties. SHP1-IN-1 exhibits aggregation post-quenching (ACQ) effect, good interference immunity and low detection limit (5.55 μM) .
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- HY-W127832
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Fluorescent Dye
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Others
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Ru(dpp)3(PF6)2 is a complex containing metallic ruthenium (Ru) and is a chemiluminescent oxygen probe. [Ru(dpp)3] 2+ is a luminescent substance that can undergo metal-to-ligand charge transfer (MLCT), and fluorescence will appear during the transfer of Ru to the ligand. At the same time, the fluorescence of Ru(dpp)3(PF6)2 is effectively quenched by molecular oxygen, so it is widely used in biosensors, environmental monitoring, and materials science. The property of Ru(dpp)3(PF6)2, which oxidizes Ru 2+ to Ru 3+, has also been used to design and construct solid-state light-emitting electrochemical cells .
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- HY-N3103
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Ethyl (E)-p-hydroxycinnamate; Ethyl trans-4-hydroxycinnamate
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Tyrosinase
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Inflammation/Immunology
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p-Coumaric Acid Ethyl Ester (Ethyl (E)-p-hydroxycinnamate; Ethyl trans-4-hydroxycinnamate) is a non-competitive, reversible inhibitor of tyrosinase (IC50=4.89 μg/mL, Ki=1.83 μg/mL), which can quench the intrinsic fluorescence of the enzyme. p-Coumaric Acid Ethyl Ester changes the binding affinity of L-tyrosine by inducing conformational changes in the catalytic domain of tyrosinase, and does not bind to the copper ion of the enzyme. p-Coumaric Acid Ethyl Ester is used in the development of medicines, cosmetics and fruit preservation products using pollen .
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- HY-123630
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FD&C RED NO. 40; CI 16035
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5-HT Receptor
TNF Receptor
IFNAR
Reactive Oxygen Species (ROS)
Interleukin Related
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Inflammation/Immunology
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Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety .
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- HY-172309
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Fluorescent Dye
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Others
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UBQ-3 NHS Ester is a fluroescent agent with a terminal NHS ester group. UBQ-3 NHS Ester has a wide quenching range from 620-730 nm, which makes the compound useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with far-red to near-IR emitting dyes such as Cy5, Cy5.5, Alexa Fluor 633, 647, 700. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
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- HY-W127716
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Fluorescent Dye
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Cancer
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Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 is a potent ruthenium-based dye. Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 can bu used as an effective quencher of quantum dots (QDs) fluorescence and the capture probe of virus antigen EV71. Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 can be used sensitive electrogenerated chemiluminescence (ECL) labels for detection of matrix metalloproteinases (MMPs) .
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- HY-D1555
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Cy7 DBCO
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Fluorescent Dye
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Others
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Cyanine7 DBCO (Cy7 DBCO) is a near-infrared fluorescent dye and an efficient bio-orthogonal quencher. Cyanine7 DBCO is formed by covalent connection of the near-infrared fluorescent dye Cy7 and the dibenzocyclooctyne (DBCO) functional group. After reacting with N3-Cy5-COOH, the fluorescence of Cy5 decreases by 90% within 90 minutes, and rapid signal attenuation can be observed within 2-5 minutes. Cyanine7 DBCO can be used for the study of deep tissue imaging and receptor-targeted therapeutic strategies .
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- HY-126823
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PGSK diacetate (5/6-mixture)
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Fluorescent Dye
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Others
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Phen green SK (PGSK) diacetate (PGSK diacetate (5/6-mixture)) is a metal ion-sensitive fluorescent probe that can penetrate cell membranes. Phen green SK (PGSK) diacetate can react with a variety of metal ions, including Fe 2+, Cd 2+, Co 2+, Ni 2+, Zn 2+, etc. Phen green SK (PGSK) diacetate chelates Fe 2+, resulting in fluorescence quenching, which can be restored when a membrane-permeable chelator is added, thereby reflecting the changes in the intracellular chelatable iron pool. The excitation/emission maxima of Phen green SK diacetate are 507/532 nm, respectively .
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- HY-123630R
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FD&C RED NO. 40 (Standard); CI 16035 (Standard)
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Fluorescent Dye
Interleukin Related
5-HT Receptor
IFNAR
Reactive Oxygen Species (ROS)
Reference Standards
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Inflammation/Immunology
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Allura Red AC (Standard) is an analytical standard of Allura Red AC. This product is intended for research and analytical applications. Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety .
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- HY-43520
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Fluorescent Dye
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Others
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BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm .
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| Cat. No. |
Product Name |
Type |
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- HY-D2864
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Fluorescent Dye
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Aza-CyBz is a selective NaCl-responsive fluorescent probe. Aza-CyBz visualizes plant salt stress by forming ordered aggregates with NaCl, leading to near-infrared fluorescence quenching. Aza-CyBz acts via electrostatic interaction between the cationic cyanine dye and NaCl to form J-aggregates, causing fluorescence quenching with a detection limit of 170 μM .
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- HY-D1030
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Fluorescent Dye
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Fluorescein Biotin is used as an alternative to radioactive biotin for detecting and quantitating biotin-binding sites by either fluorescence or absorbance; the the fluorescence or absorbance of Fluorescein Biotin is quenched, upon binding to avidin or streptavidin.
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- HY-W012642
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Fluorescent Dye
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2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. When 2-Aminopurine is inserted in anoligonucleotide, its fluorescence is highly quenched by stacking with the natural bases. 2-Aminopurine has been used to probe nucleic acid structure and dynamics .
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- HY-42984
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Fluorescent Dye
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BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
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- HY-D0720
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Lucigenin
Maximum Cited Publications
7 Publications Verification
NSC-151912; L-6868
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Fluorescent Dye
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Lucigenin is a chemiluminescence probe. Lucigenin can be used to detect the production of endogenous superoxide anion radical (O 2-). Lucigenin is extremely sensitive to chloride ions, while it combined with chloride ions, the fluorescence will be quenched. Lucigenin also can be used as a chloride indicator. Ex/Em=455/505 nm .
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- HY-D2924
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Fluorescent Dye
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CBG-549-QSY7 is a Dylight 549-labeled SNAP tag fluorescent probe (Ex/Em: 546 nm/580 nm), specially designed for non-washable fluorescence imaging applications. CBG-549-QSY7 employs an intramolecular FRET quenching mechanism: intramolecular fluorescence quenching occurs before binding to the SNAP tag, and upon binding, the quenching group is cleaved, releasing fluorescence. The background fluorescence of CBG-549-QSY7 is extremely low, and clear cell membrane images can be obtained after 5 minutes of incubation .
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- HY-173308
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Fluorescent Dye
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QSY-21 is a fluorescence quencher. QSY-21 possesses broad absorption in far red and NIR range, and can quench fluorescence of dyes that emit in this region. This is a carboxylic acid derivative. QSY-21 has intense absorption maximum at 661 nm, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common quencher for Cyanine5, Cyanine5.5, AF 647, or other spectrally similar fluorescent dyes.
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- HY-W012642A
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Fluorescent Dye
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2-Aminopurine dihydrochloride is a fluorescent analog of guanosine. 2-Aminopurine dihydrochloride can be used as a fluorescence probe for nucleic acid structure and dynamics. Incorporating 2-Aminopurine dihydrochloride into DNA quenches its fluorescence .
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- HY-D2579
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Fluorescent Dye
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Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .
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- HY-118213
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Fluorescent Dye
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N-(2-Hydroxyethyl)-1,8-naphthalimide is a fluorescent probe for detecting nucleic acids and their precursors. The fluorescence of N-(2-Hydroxyethyl)-1,8-naphthalimide will be quenched by these molecules.
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- HY-W127703
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Fluorescent Dye
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Octadecyl Rhodamine B chloride is an amphiphilic fluorescent probe with both membrane-binding ability and fluorescent properties, which easily forms aggregates in aqueous phase leading to self-quenching. Octadecyl Rhodamine B chloride can be used for cell membrane staining and further applied in membrane biology research. The maximum absorption/emission wavelengths of Octadecyl Rhodamine B chloride monomer in Triton X-100 are 565 nm/585 nm; the absorption peaks of aggregates in water are blue-shifted to 524 nm and 574 nm, with significantly quenched fluorescence .
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- HY-D2761
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Fluorescent Dye
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DY-680-NHS ester is an amine reactive hydrophilic fluorochrome. It can be conjugated to an antibody with higher D/P ratio without causing fluorescence quenching and conjugate precipitation, and it is widely used in applications including western blotting, microscopy, flow cytometry, and cell-based assays. The spectrum has an excitation of 690nm and emission at 709nm.
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- HY-D1682
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Fluorescent Dye
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NFQ1 is a non-fluorescent quencher (absorption wavelength: 474 nm), and is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method. OS-FRET enable measurement of unquenched donor emission in the same sample. NFQ1 shows broad absorption spectrum ensuring its utility as a dark acceptor for many donors .
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- HY-P3749
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Fluorescent Dye
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Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage .
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- HY-D2365
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Fluorescent Dye
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QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in
the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules .
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- HY-D2735
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Fluorescent Dye
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QSY-9-succinimidyl ester is a nonfluorescent acceptor molecule with a terminal NHS ester group. QSY-9-succinimidyl ester has a wide and intense quenching range from 500-600 nm that makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 500 nm to 600 nm. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
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- HY-D2204
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Fluorescent Dye
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SHP1-IN-1 (compound 5p) is a fluorescent probe for the protein tyrosine phosphatase SHP1 containing the Src homology 2 domain. SHP1-IN-1 has SHP1 inhibitory activity, selectivity for Fe 3+ ions and good fluorescence properties. SHP1-IN-1 exhibits aggregation post-quenching (ACQ) effect, good interference immunity and low detection limit (5.55 μM) .
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- HY-123630
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FD&C RED NO. 40; CI 16035
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Fluorescent Dye
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Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety .
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- HY-W127716
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Fluorescent Dye
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Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 is a potent ruthenium-based dye. Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 can bu used as an effective quencher of quantum dots (QDs) fluorescence and the capture probe of virus antigen EV71. Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 can be used sensitive electrogenerated chemiluminescence (ECL) labels for detection of matrix metalloproteinases (MMPs) .
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- HY-D1555
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Cy7 DBCO
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Fluorescent Dye
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Cyanine7 DBCO (Cy7 DBCO) is a near-infrared fluorescent dye and an efficient bio-orthogonal quencher. Cyanine7 DBCO is formed by covalent connection of the near-infrared fluorescent dye Cy7 and the dibenzocyclooctyne (DBCO) functional group. After reacting with N3-Cy5-COOH, the fluorescence of Cy5 decreases by 90% within 90 minutes, and rapid signal attenuation can be observed within 2-5 minutes. Cyanine7 DBCO can be used for the study of deep tissue imaging and receptor-targeted therapeutic strategies .
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- HY-123630R
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FD&C RED NO. 40 (Standard); CI 16035 (Standard)
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Fluorescent Dye
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Allura Red AC (Standard) is an analytical standard of Allura Red AC. This product is intended for research and analytical applications. Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety .
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- HY-43520
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Fluorescent Dye
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BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells. BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm .
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| Cat. No. |
Product Name |
Type |
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- HY-W142692
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Biochemical Assay Reagents
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Dodecyl β-D-glucopyranoside is a non-ionic detergent and surfactant commonly used to solubilize and purify membrane proteins in biochemical research. Dodecyl β-D-glucopyranoside also interacts with bovine serum albumin (BSA) to quench its intrinsic fluorescence. The critical micelle concentration (CMC) of Dodecyl β-D-glucopyranoside (DG) and DG/BSA complex is 2.0 mM and 2.34 mM, respectively. Micelles can be formed in aqueous solutions above this concentration .
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- HY-W127832
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Biochemical Assay Reagents
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Ru(dpp)3(PF6)2 is a complex containing metallic ruthenium (Ru) and is a chemiluminescent oxygen probe. [Ru(dpp)3] 2+ is a luminescent substance that can undergo metal-to-ligand charge transfer (MLCT), and fluorescence will appear during the transfer of Ru to the ligand. At the same time, the fluorescence of Ru(dpp)3(PF6)2 is effectively quenched by molecular oxygen, so it is widely used in biosensors, environmental monitoring, and materials science. The property of Ru(dpp)3(PF6)2, which oxidizes Ru 2+ to Ru 3+, has also been used to design and construct solid-state light-emitting electrochemical cells .
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- HY-170466
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1-Palmitoyl-2-stearoyl-(12-doxyl)-sn-glycero-3-phosphocholine
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Biochemical Assay Reagents
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16:0-12 Doxyl PC (1-Palmitoyl-2-stearoyl-(12-doxyl)-sn-glycero-3-phosphocholine) is a fluorescence quencher, and its nitrogen oxide is located downstream of the sn-2 chain. 16:0-12 Doxyl PC has a better fluorescence quenching effect onthe fluorophore is buried within the hydrocarbon interior of the bilayer .
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| Cat. No. |
Product Name |
Target |
Research Area |
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- HY-P4920
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Fluorescent Dye
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Others
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Mca-SEVNLDAEFK(Dnp)-NH2 contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence, such as can be used to measure the activity of BACE-1 .
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- HY-P10461
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Peptides
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Others
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Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp is a fluorescence-quenched peptide substrate for human proteinase 3 (kcat/Km = 1,570 mM -1s -1), and can be used for detection of proteinase 3 (PR3) activity .
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- HY-P3749
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Fluorescent Dye
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Others
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Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage .
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- HY-P3123A
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Fluorescent Dye
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Others
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Dnp-RPLALWRS TFA is a fluorescent peptide substrate designed for human matrilysin (MMP-7). After enzymatic cleavage of Dnp-RPLALWRS TFA at the alanine-leucine bond, the release of the Dnp group alleviates fluorescence quenching, thereby enabling real-time quantitative analysis of MMP-7 activity by increasing tryptophan emission. Dnp-RPLALWRS TFA provides a sensitive and efficient detection method for kinetic studies and inhibitor screenin .
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- HY-P5485
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Bacterial
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Others
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Dabcyl-QALPETGEE-Edans is a synthetic peptide substrate for fluorescence resonance energy transfer (FRET) detection of Sortase A (SrtA) enzymatic activity. Dabcyl-QALPETGEE-Edans is labeled at both ends with the fluorescent group Edans (donor) and the quencher Dabcyl (acceptor), respectively. Dabcyl-QALPETGEE-Edans's central sequence contains a conserved motif specifically recognized by SrtA. Dabcyl-QALPETGEE-Edans demonstrates high sensitivity in vitro assays, enabling precise determination of the half-maximal inhibitory concentration of SrtA .
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- HY-P4919
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Beta-secretase
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Others
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Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
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- HY-P10922
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Cathepsin
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Others
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Cathepsin E substrate e is a substrate of Cathepsin E. Cathepsin E substrate e was designed in such a way that due to the close proximity of a Mca-donor and a Dnp-acceptor, a near complete intramolecular quenching effect was achieved in its intact state. After the proteolytic cleavage of the hydrophobic motif of the peptide substrate, both Mca and Dnp would be further apart, resulting in bright fluorescence .
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- HY-P3123
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Fluorescent Dye
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Others
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Dnp-RPLALWRS is a fluorescent peptide substrate designed for human matrilysin (MMP-7). After enzymatic cleavage of Dnp-RPLALWRS at the alanine-leucine bond, the release of the Dnp group alleviates fluorescence quenching, thereby enabling real-time quantitative analysis of MMP-7 activity by increasing tryptophan emission. Dnp-RPLALWRS provides a sensitive and efficient detection method for kinetic studies and inhibitor screenin .
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- HY-P11302
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CHIKV
Virus Protease
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Infection
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DABCYL-RAGGYIFS-(E-EDANS)-NH2, an octapeptide, is a FRET-based substrate peptide of CHIKV nsP2protease with highly sensitivity. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can be cleaved by the active CHIKV nsP2protease, separating fluorophore and quencher and resulting in an increase of fluorescence signal. DABCYL-RAGGYIFS-(E-EDANS)-NH2 can used for the determination of CHIKV nsP2protease activity .
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- HY-K1042
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Maximum Cited Publications
21 Publications Verification
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MCE AntiFade Mounting Medium can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect.
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-
- HY-K1048
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5 Publications Verification
|
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AntiFade Reagent (with DAPI) is used to slow the fluorescence quenching.
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-
- HY-K1047
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AntiFade Mounting Medium (with DAPI) is a sealing reagent used to slow the fluorescence quenching.
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| Cat. No. |
Product Name |
Category |
Target |
Chemical Structure |
| Cat. No. |
Product Name |
|
Classification |
-
- HY-D1555
-
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Cy7 DBCO
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DBCO
|
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Cyanine7 DBCO (Cy7 DBCO) is a near-infrared fluorescent dye and an efficient bio-orthogonal quencher. Cyanine7 DBCO is formed by covalent connection of the near-infrared fluorescent dye Cy7 and the dibenzocyclooctyne (DBCO) functional group. After reacting with N3-Cy5-COOH, the fluorescence of Cy5 decreases by 90% within 90 minutes, and rapid signal attenuation can be observed within 2-5 minutes. Cyanine7 DBCO can be used for the study of deep tissue imaging and receptor-targeted therapeutic strategies .
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-
- HY-D2579
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DBCO
|
|
Sulfo DBCO-UBQ-2 is a click chemistry reagent combining a dark quencher, UBQ-2, with a polyaromatic-azo backbone, offering no native emission. UBQ-2 effectively quenches fluorescence in the 560-670 nm range, ideal for qPCR probes and FRET applications with orange to far-red dyes .
|
| Cat. No. |
Product Name |
|
Classification |
-
- HY-W013195
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|
Nucleoside Analogs
Adenosine
|
|
8-Bromoadenosine is an adenosine analog. Adenosine analogs mostly act as smooth muscle vasodilators and have also been shown to inhibit cancer progression. 8-Bromoadenosine can quench the intrinsic fluorescence of human serum albumin (HSA) through static quenching procedure. 8-Bromoadenosine can be used to synthesize adenosine phosphate, such as Acadesine (HY-13417), Clofarabine (HY-A0005), Fludarabine phosphate (HY-B0028) and Vidarabine (HY-B0277) .
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-
- HY-160062
-
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Aptamers
|
|
S2.2 aptamer sodium is a nucleic acid aptamer targeting the mucin MUC1 and can be used for targeted imaging of MCF-7 cancer cells. S2.2 aptamer sodium was labeled with Cy5, and when fluorescent silicon nanodots (SiND) were present, the fluorescence was quenched; when MUC1 was also present, the fluorescence was restored. S2.2 aptamer sodium detects MUC1 with a linear range of 3.33-250 nM .
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