2. GPCR/G Protein MAPK/ERK Pathway PI3K/Akt/mTOR Apoptosis NF-κB Metabolic Enzyme/Protease
  3. Ras PI3K Akt Caspase Apoptosis Bcl-2 Family NF-κB MMP
  4. Salvianolic acid F

Salvianolic acid F is a KRAS inhibitor, especially for KRAS G12D. Salvianolic acid F inhibits NF-kB, MMP-9, and NO simultaneously. Salvianolic acid F inhibits cancer cell growth, invasion, and migration and induces apoptosis via the EP300/PI3K/AKT pathway in vitro. Salvianolic acid F inhibits the growth of KRAS-dependent lung cancer cells via the PI3K/AKT signaling pathway in vivo. Salvianolic acid F can be used in the research of various cancers, including KRAS G12D-driven non-small cell lung cancer (NSCLC) and ovarian cancer.

For research use only. We do not sell to patients.

Salvianolic acid F

Salvianolic acid F Chemical Structure

CAS No. : 158732-59-3

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Based on 1 publication(s) in Google Scholar

Salvianolic acid F Related Antibodies

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K-Ras H-Ras N-Ras Ras

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Akt Akt1 Akt2 Akt3

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

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  • Purity & Documentation

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Description

Salvianolic acid F is a KRAS inhibitor, especially for KRAS G12D. Salvianolic acid F inhibits NF-kB, MMP-9, and NO simultaneously. Salvianolic acid F inhibits cancer cell growth, invasion, and migration and induces apoptosis via the EP300/PI3K/AKT pathway in vitro. Salvianolic acid F inhibits the growth of KRAS-dependent lung cancer cells via the PI3K/AKT signaling pathway in vivo. Salvianolic acid F can be used in the research of various cancers, including KRAS G12D-driven non-small cell lung cancer (NSCLC) and ovarian cancer[1][2].

IC50 & Target[1][2]

K-RAS

 

Caspase 3

 

MMP-9

 

In Vitro

Salvianolic acid F (0-100 μM, 24-72 h) shows cytotoxicity against A549, OE-KRAS A549 cells, with 24 h IC50s of 41.18, 36.55 μM, with 48 h IC50s of 35.17, 29.33 μM, shows cytotoxicity against OVCAR-3, SK-OV-3 OC cells, with IC50s of 28.89, 29.94 µM[1][2].
Salvianolic acid F (0-40 μM, 48 h) inhibits migration and proliferation and promotes apoptosis in OE-KRAS A549, OVCAR-3, SK-OV-3 OC cells[1][2].
Salvianolic acid F (0-40 μM, 48 h) suppresses the EP300/PI3K/AKT signaling pathway in OVCAR-3, SK-OV-3 OC cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Salvianolic acid F Related Antibodies

Cell Migration Assay [1]

Cell Line: OE-Ctrl, OE-KRAS, shCtrl, and shKRAS A549 cells
Concentration: 0, 10, 20 µM
Incubation Time: 48 h
Result: Inhibited the in vitro migration of OE-KRAS A549 cells, but had a poorer inhibition on the migration of shKRAS A549 cells at 20 µM.

Apoptosis Analysis[1]

Cell Line: OE-KRAS and shKRAS A549 cells
Concentration: 0, 10, 20 µM
Incubation Time: 48 h
Result: Induced marked apoptosis in OE-KRAS A549 cells, but showed not obvious apoptosis in shKRAS A549 cells.

Immunofluorescence[2]

Cell Line: OVCAR-3, SK-OV-3 OC cells
Concentration: 0, 20, 40 µM
Incubation Time: 48 h
Result: Significant reduced EdU-positive cells.

Cell Migration Assay [2]

Cell Line: OVCAR-3, SK-OV-3 OC cells
Concentration: 0, 20, 40 µM
Incubation Time: 48 h
Result: Inhibited cell migration.

Apoptosis Analysis[2]

Cell Line: OVCAR-3, SK-OV-3 OC cells
Concentration: 0, 20, 40 µM
Incubation Time: 48 h
Result: Exhibited a significantly elevated apoptosis rate in a dose-dependent manner compared to untreated cells.
Increased caspase-3 cleavage, Bax expression, decreased Bcl-2 expression in a dose-dependent manner compared to control cells.

Western Blot Analysis[2]

Cell Line: OVCAR-3, SK-OV-3 OC cells
Concentration: 0, 20, 40 µM
Incubation Time: 48 h
Result: Decreased EP300, p-PI3K/PI3K, Bax/Bcl-2, and cleaved caspase-3/caspase-3 ratios.
In Vivo

Salvianolic acid F (10-20 mg/kg, i.p., each day for 15 days) is effective in inhibiting KRAS-driven lung tumor growth by influencing the downstream PI3K/AKT pathway in A549 xenografts nude mice model[1].
Salvianolic acid F (10-20 mg/kg, i.p., every 2 days for 40 days) inhibits lung tumor growth by influencing the downstream PI3K/AKT pathway in KrasG12D mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A549 (5 × 106) xenografts nude mice (6 weeks old BALB/c) model[1]
Dosage: 10, 20 mg/kg
Administration: i.p., each day for 15 days
Result: Did not induce significant weight loss and began to inhibit tumor growth from day 7 at 20 mg/kg, with no significant inhibitory effect at 10 mg/kg.
Inhibited the expression of KRAS and reduced cell proliferation in mice at 20 mg/kg.
Inhibited AKT phosphorylation, increased the expression of Bax, cleaved-Caspase3 and cleaved-PARP, decreased the expression of Bcl2, promoted apoptosis in lung cancer cells.
Animal Model: KrasG12D mice model[1]
Dosage: 10, 20 mg/kg
Administration: i.p., every 2 days for 40 days
Result: Not changed body weight, decreased the number and size of lung tumors, reaching 48% and 64% in the 10 mg/kg and 20 mg/kg groups, compared with the control group.
Inhibited KrasG12D expression, inhibited the pathological histomorphology, reduced cell proliferation.
Inhibited AKT phosphorylation, increased the expression of Bax, cleaved-Caspase3 and cleaved-PARP, decreased the expression of Bcl2, promoted apoptosis in lung cancer cells.
Molecular Weight

314.29

Formula

C17H14O6
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)/C=C/C1=CC=C(O)C(O)=C1/C=C/C2=CC=C(O)C(O)=C2
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (318.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1818 mL 15.9089 mL 31.8177 mL
5 mM 0.6364 mL 3.1818 mL 6.3635 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator
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Purity & Documentation

Purity: 99.30%

SDS (251 KB)

COA (250 KB) HNMR (134 KB) CNMR (148 KB) LCMS (339 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1818 mL 15.9089 mL 31.8177 mL 79.5444 mL
5 mM 0.6364 mL 3.1818 mL 6.3635 mL 15.9089 mL
10 mM 0.3182 mL 1.5909 mL 3.1818 mL 7.9544 mL
15 mM 0.2121 mL 1.0606 mL 2.1212 mL 5.3030 mL
20 mM 0.1591 mL 0.7954 mL 1.5909 mL 3.9772 mL
25 mM 0.1273 mL 0.6364 mL 1.2727 mL 3.1818 mL
30 mM 0.1061 mL 0.5303 mL 1.0606 mL 2.6515 mL
40 mM 0.0795 mL 0.3977 mL 0.7954 mL 1.9886 mL
50 mM 0.0636 mL 0.3182 mL 0.6364 mL 1.5909 mL
60 mM 0.0530 mL 0.2651 mL 0.5303 mL 1.3257 mL
80 mM 0.0398 mL 0.1989 mL 0.3977 mL 0.9943 mL
100 mM 0.0318 mL 0.1591 mL 0.3182 mL 0.7954 mL
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Salvianolic acid F Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Salvianolic acid F158732-59-3RasPI3KAktCaspaseApoptosisBcl-2 FamilyNF-κBMMPPhosphoinositide 3-kinasePKBProtein kinase BNuclear factor-κBNuclear factor-kappaBMatrix metalloproteinasesInhibitorapoptosisA549 cellsOVCAR-3 cellsSK-OV-3 OC cellsKrasG12D mice modellung cancerovarian cancerinhibitorinhibit

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