OTUD3 Protein, Human

OTUD3 Protein, Human is the recombinant human-derived OTUD3, expressed by E. coli , with tag Free labeled tag. ,

OTUD3 is a deubiquitinating enzyme that hydrolyzes Lys-6- and Lys-11-linked polyubiquitin chains, as well as heterotypic (mixed and branched) and homotypic chains. It serves as a critical regulator of energy metabolism, with glucose and fatty acids triggering its nuclear translocation via CBP-dependent acetylation. In the nucleus, OTUD3 deubiquitinates and stabilizes the nuclear receptor PPARD, regulating the expression of genes involved in glucose and lipid metabolism and oxidative phosphorylation. Additionally, OTUD3 acts as a negative regulator of ribosome quality control (RQC) by mediating the deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby counteracting ZNF598-mediated 40S ubiquitination.

Human

E. coli

Tag Free

Q5T2D3 (E52-D209)

23252

Partial

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.