2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. Chemokine & Receptors
  5. CC Chemokines
  6. MCP-1/CCL2
  7. MCP-1/CCL2 Protein, Human (HEK293, hFc)

MCP-1/CCL2 (human) acts as a ligand for CCR2, triggering a potent chemotactic response and intracellular calcium mobilization upon CCR2 binding. It specifically attracts monocytes and basophils while sparing neutrophils and eosinophils. MCP-1/CCL2 Protein, Human (HEK293, hFc) is the recombinant human-derived MCP-1/CCL2 protein, expressed by HEK293, with C-hFc labeled tag.

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Description

MCP-1/CCL2 (human) acts as a ligand for CCR2, triggering a potent chemotactic response and intracellular calcium mobilization upon CCR2 binding. It specifically attracts monocytes and basophils while sparing neutrophils and eosinophils. MCP-1/CCL2 Protein, Human (HEK293, hFc) is the recombinant human-derived MCP-1/CCL2 protein, expressed by HEK293, with C-hFc labeled tag.
Background

MCP-1/CCL2, Human, serves as a ligand for C-C chemokine receptor CCR2, initiating a robust chemotactic response and intracellular calcium mobilization upon CCR2 binding and activation. This chemokine exhibits chemotactic activity specifically for monocytes and basophils, excluding neutrophils or eosinophils from its influence. Additionally, MCP-1/CCL2 may play a role in recruiting monocytes to the arterial wall during the progression of atherosclerosis. Existing in equilibrium as a monomer or homodimer, it is anchored to endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Furthermore, MCP-1/CCL2 interacts with TNFAIP6 through its Link domain.
Species

Human
Source

HEK293
Tag

C-hFc
Accession

P13500 (Q24-T99)
Gene ID

6347
Molecular Construction
N-term
CCL2 (Q24-T99)
Accession # P13500
hFc
C-term
Protein Length

Full Length of Mature Protein
Synonyms
C-C motif chemokine 2; CCL2; HC11; MCAF; MCP-1; HSMCR30; MCP1; SCYA2; SMC-CF; GDCF-2
Predicted Molecular Mass
35.1 kDa
Molecular Weight

Approximately 40-50 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MCP-1/CCL2 Protein, Human (HEK293, hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MCP-1/CCL2 Protein, Human (HEK293, hFc)
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