2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. Chemokine & Receptors
  5. CC Chemokines
  6. CCL1
  7. I-309/CCL1 Protein, Human (HEK293, mFc)

I-309/CCL1 Protein, Human (HEK293, mFc) is the recombinant human-derived I-309/CCL1 protein, expressed by HEK293, with C-mFc tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Description

I-309/CCL1 Protein, Human (HEK293, mFc) is the recombinant human-derived I-309/CCL1 protein, expressed by HEK293, with C-mFc tag.
Background

I-309/CCL1 is a cytokine that exhibits chemotactic activity for monocytes but not for neutrophils. It functions by binding to the CCR8 receptor.
Species

Human
Source

HEK293
Tag

C-mFc
Accession

P22362 (K24-K96)
Gene ID

6346
Molecular Construction
N-term
CCL1 (K24-K96)
Accession # P22362
mFc
C-term
Synonyms
C-C Motif Chemokine 1; Small-Inducible Cytokine A1; T Lymphocyte-Secreted Protein I-309; CCL1; SCYA1
Predicted Molecular Mass
35.4 kDa.
Molecular Weight

Approximately 42-45 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

I-309/CCL1 Protein, Human (HEK293, mFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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I-309/CCL1 Protein, Human (HEK293, mFc)
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