2. Membrane Transporter/Ion Channel Neuronal Signaling Anti-infection Immunology/Inflammation NF-κB MAPK/ERK Pathway Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. TRP Channel Bacterial Fungal NO Synthase COX NF-κB p38 MAPK Keap1-Nrf2 Heme Oxygenase (HO) PERK JNK Calcium Channel
  4. Pulegone

Pulegone is a monoterpene ketone compound widely present in the essential oils of many plants. Pulegone can also be used as a bird repellent. Pulegone has multiple activities such as anti-inflammatory, antibacterial, antifungal, and anti-hyperalgesic effects. Pulegone is particularly effective against bacteria of the Salmonella species.

For research use only. We do not sell to patients.

Pulegone Chemical Structure

Pulegone Chemical Structure

CAS No. : 89-82-7

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Liquid
500 mg In-stock
1 g In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Pulegone:

Pulegone Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Pulegone

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TRPC TRPM TRPV TRPA TRPML

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nNOS iNOS eNOS

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COX COX-1 COX-2 COX-3

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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HO-1

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JNK1 JNK2 JNK3 JNK

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pulegone is a monoterpene ketone compound widely present in the essential oils of many plants. Pulegone can also be used as a bird repellent. Pulegone has multiple activities such as anti-inflammatory, antibacterial, antifungal, and anti-hyperalgesic effects. Pulegone is particularly effective against bacteria of the Salmonella species[1][2][3][4][5].

In Vitro

Pulegone (6.25-50 µg/mL; 20-26 h) can inhibit the expression of inflammatory mediators by downregulating the iNOS, COX-2, NF-κB, and MAPK signaling pathways and upregulating Nrf-2/HO-1 in LPS (HY-D1056)-stimulated RAW264.7 cells[1].
Pulegone (0.01-3 mM; 0-5 min) can increase the intracellular Ca2+ concentration in chicken sensory neurons[2].
Pulegone (0.01-3 mM; 0-5 min) can activate TRPM8 and TRPA1 and inhibit TRPM8 at high concentrations (1 mM and above) in HEK293 cells expressing chicken TRPM8, TRPA1, and TRPV1[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pulegone Related Antibodies

Western Blot Analysis[1]

Cell Line: LPS (HY-D1056)-stimulated RAW264.7 cells.
Concentration: 6.25, 12.5, 25 and 50 µg/mL
Incubation Time: Pretreated with 2 h, then co-incubation for 18 h
Result: Inhibited the expression of iNOS, COX-2 and NF-κB.
Downregulated phosphorylation of ERK, JNK, and p38.
In Vivo

Pulegone (100 mM; intraplantar injection; single dose) can induce pain-related behaviors in chicks, but these behaviors can be inhibited by a TRPA1 antagonist[2].
Pulegone (10-150 mg/kg; intraperitoneal injection; single dose) has a beneficial effect in a rat model of inflammatory pain[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (300 g) treated Complete Freund's adjuvant (HY-153808)[3].
Dosage: 10, 50, 100 and 150 mg/kg
Administration: Intraperitoneal injection; single dose
Result: Exerted an anti-hyperalgesic effect, increased the mechanical threshold, thermal heat threshold and decreased the thermal cold score at dose of 100 mg/kg.
Was devoid of in vivo locomotor side effects.
Molecular Weight

152.24

Formula

C10H16O
CAS No.
Appearance

Liquid (Density: 0.937 g/cm3)

Color

Colorless to light yellow

SMILES

O=C1/C(CC[C@@H](C)C1)=C(C)/C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 270 mg/mL (1773.52 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5686 mL 32.8429 mL 65.6858 mL
5 mM 1.3137 mL 6.5686 mL 13.1372 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.25 mg/mL (14.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.25 mg/mL (14.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.5686 mL 32.8429 mL 65.6858 mL 164.2144 mL
5 mM 1.3137 mL 6.5686 mL 13.1372 mL 32.8429 mL
10 mM 0.6569 mL 3.2843 mL 6.5686 mL 16.4214 mL
15 mM 0.4379 mL 2.1895 mL 4.3791 mL 10.9476 mL
20 mM 0.3284 mL 1.6421 mL 3.2843 mL 8.2107 mL
25 mM 0.2627 mL 1.3137 mL 2.6274 mL 6.5686 mL
30 mM 0.2190 mL 1.0948 mL 2.1895 mL 5.4738 mL
40 mM 0.1642 mL 0.8211 mL 1.6421 mL 4.1054 mL
50 mM 0.1314 mL 0.6569 mL 1.3137 mL 3.2843 mL
60 mM 0.1095 mL 0.5474 mL 1.0948 mL 2.7369 mL
80 mM 0.0821 mL 0.4105 mL 0.8211 mL 2.0527 mL
100 mM 0.0657 mL 0.3284 mL 0.6569 mL 1.6421 mL
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Pulegone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pulegone89-82-7TRP ChannelBacterialFungalNO SynthaseCOXNF-κBp38 MAPKKeap1-Nrf2Heme Oxygenase (HO)PERKJNKCalcium ChannelTransient receptor potential channelsNitric oxide synthasesNOSCyclooxygenaseNuclear factor-κBNuclear factor-kappaBHeme OxygenaseProtein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinasec-Jun N-terminal kinaseCa2+ channelsCa channelsinflammatory pain model RAW264.7 cellschicken sensory neuronsInhibitorinhibitorinhibit

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