Neocryptotanshinone

Name: Neocryptotanshinone
Brand: MedChemExpress
SKU: HY-119720
Rating: 4.5 (1 reviews)

Cat. No.: HY-119720 Purity: 95.07%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Neocryptotanshinone, a fatty diterpenoids from Salvia Miltiorrhiza, inhibits lipopolysaccharide-induced inflammation by suppression of NF-κB and iNOS signaling pathways.

For research use only. We do not sell to patients.

Neocryptotanshinone Chemical Structure

CAS No. : 109664-02-0

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

View All Isoforms

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All NO Synthase Isoform Specific Products:

View All Isoforms

nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

Neocryptotanshinone, a fatty diterpenoids from Salvia Miltiorrhiza, inhibits lipopolysaccharide-induced inflammation by suppression of NF-κB and iNOS signaling pathways^[1]^[2].

IC₅₀ & Target

iNOS

In Vitro

Neocryptotanshinone exhibits anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways^[1].
Neocryptotanshinone (10 μM and 20 μM, 24 h) inhibits LPS-induced iNOS protein expression in RAW264.7 cells^[1].
Neocryptotanshinone (20 μM, 24 h) shows no obvious cytotoxic effect towards murine RAW264.7 macrophages^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	RAW264.7 cells
Concentration:	5, 10 and 20 μM
Incubation Time:	24 hours
Result:	Inhibited LPS-induced cell viability in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	RAW264.7 cells
Concentration:	20 μM
Incubation Time:	24 hours
Result:	Inhibited LPS-induced activation of NF-κB pathway and down-regulated LPS-induced expression of p-NF-κB p65, p-IκBα and p-IKKβ.

In Vivo

Neocryptotanshinone causes reversals of decreased pain thresholds induced by MSU treatment after 30, 60, and 120 min S. miltiorrhiza Bunge extract treatment (contains single active components)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	MSU-induced pain model in male ICR mice (weighing 20-25 g)^[2]
Dosage:	10, 25, 50 or 100 mg/kg
Administration:	Oral gavage; for 30, 60, 120 min
Result:	Inhibited inflammatory symptoms and nociceptive behaviors in a dose-dependent manner.

Molecular Weight

314.38

Formula

C₁₉H₂₂O₄

CAS No.

109664-02-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C([C@@H](C)CO)=C(O)C(C2=C1C=CC3=C2CCCC3(C)C)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (318.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1809 mL	15.9043 mL	31.8086 mL
5 mM	0.6362 mL	3.1809 mL	6.3617 mL
10 mM	0.3181 mL	1.5904 mL	3.1809 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.82%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (249 KB) HNMR (154 KB) RP-HPLC (244 KB) MS (78 KB)

Handling Instructions (2659 KB)

References

[1]. Chuanhong Wu, et al. Neocryptotanshinone Inhibits Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages by Suppression of NF-κB and iNOS Signaling Pathways. Acta Pharm Sin B. 2015 Jul;5(4):323-9. [Content Brief]

[2]. H C Lin, et al. Two New Fatty Diterpenoids From Salvia Miltiorrhiza. J Nat Prod. 2001 May;64(5):648-50. [Content Brief]

[3]. FENG Jinghui, et al. Effects of Salvia miltiorrhiza Bunge extract and its single components on monosodium urate-induced pain in vivo and lipopolysaccharide-induced inflammation in vitro. J Tradit Chin Med. 2021. 41(2): 219-226. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1809 mL	15.9043 mL	31.8086 mL	79.5216 mL
	5 mM	0.6362 mL	3.1809 mL	6.3617 mL	15.9043 mL
	10 mM	0.3181 mL	1.5904 mL	3.1809 mL	7.9522 mL
	15 mM	0.2121 mL	1.0603 mL	2.1206 mL	5.3014 mL
	20 mM	0.1590 mL	0.7952 mL	1.5904 mL	3.9761 mL
	25 mM	0.1272 mL	0.6362 mL	1.2723 mL	3.1809 mL
	30 mM	0.1060 mL	0.5301 mL	1.0603 mL	2.6507 mL
	40 mM	0.0795 mL	0.3976 mL	0.7952 mL	1.9880 mL
	50 mM	0.0636 mL	0.3181 mL	0.6362 mL	1.5904 mL
	60 mM	0.0530 mL	0.2651 mL	0.5301 mL	1.3254 mL
	80 mM	0.0398 mL	0.1988 mL	0.3976 mL	0.9940 mL
	100 mM	0.0318 mL	0.1590 mL	0.3181 mL	0.7952 mL