Estradiol hemihydrate (Synonyms: β-Estradiol hemihydrate; 17β-Estradiol hemihydrate; 17β-Oestradiol hemihydrate)

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Estradiol (β-Estradiol) hemihydrate is a steroid hormone and the major female sex hormone. Estradiol (β-Estradiol) hemihydrate can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol (β-Estradiol) hemihydrate can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering.

For research use only. We do not sell to patients.

Estradiol hemihydrate Chemical Structure

CAS No. : 35380-71-3

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Other In-stock Forms of Estradiol hemihydrate:

Estradiol

Other Forms of Estradiol hemihydrate:

Estradiol In-stock

87 Publications Citing Use of MCE Estradiol hemihydrate

Cell Viability Assay

RT-PCR

: Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2025 Feb;16(1):e13693. [Abstract]; Estradiol (E2) (10-100 nM) exhibites an increase in the SIRT1 protein level in C2C12 cells, without significantly affecting the mRNA level.

: Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 Jul 6:947:174543. [Abstract]; Estradiol (E2) (1 μM, 8 h) alone or in combination with TEB (100 μM, 8 h) (TEB + E2) significantly increases the protein expression levels of FoxL2 and pFoxL2 (Ser238) in ZF4 cells.

: Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Aug 7:e14303. [Abstract]; Estradiol (3-25 ng/mouse; s.c.; for 7 consecutive days) significantly decreases LAMIN A levels while increasing PROGERIN and PRELAMIN A levels in the uteri of mice injected with 10 ng or 25 ng, compared to those receiving the 3 ng dose.

: Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2023 Mar 10;236:123987. [Abstract]; Estradiol (1-100 nM; 6-72 h) promotes the proliferation of sheep myoblasts.

: Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Aug 29;10(24):10874-10891. [Abstract]; Estradiol (E2) (100 nM; 36 h) treatment can enhance the expression of ERRα and VLDL-related genes such as Apob, Mttp and Pla2g12b, whereas the induction is blocked with loss of ERRα in primary hepatocytes.

: Estradiol hemihydrate purchased from MedChemExpress. Usage Cited in: Environ Pollut. 2021 Feb 1;270:116015. [Abstract]; Estradiol (1-10 nM; 24 h) significantly induces mRNA expression of both pS2 and NRF1 genes in MCF-7 cells.

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Description

In Vitro

Estradiol hemihydrate (10 nM, 7 days) induces neural differentiation and increased neurite branching of human endometrial stem cells (hEnSCs)^[1].
Estradiol hemihydrate (17β-estradiol, 10 nM, 7 days) increases the expression of neuron-like cell markers (Tuj-1, nestin and NF-H) in neural-like cells differentiated from hEnSCs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[1]

Cell Line:	Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration:	10 nM
Incubation Time:	7 days
Result:	Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.

Immunofluorescence^[1]

Cell Line:	Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration:	10 nM
Incubation Time:	7 days
Result:	Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.

In Vivo

Estradiol hemihydrate (1 nM, the hippocampal slices from FBN-ARO-KO mice) rescues long-term potentiation (LTP) amplitude^[1].
Estradiol hemihydrate (0.0167 mg, implanted s.c., FBN-ARO-KO mice) rescues the molecular and functional deficits in FBN-ARO-KO mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	FBN-ARO-KO Mice^[2]
Dosage:	1 nM
Administration:	Treated for the hippocampal slices
Result:	Rescued long-term potentiation (LTP) amplitude of both male and female mice.

Animal Model:	FBN-ARO-KO Mice^[2]
Dosage:	0.0167 mg
Administration:	Alzet minipumps with Estradiol (implanted s.c.), examined 7 days after minipump implantation.
Result:	Restored hippocampal and cortical E2 levels to 93%, phosphorylation of AKT, ERK and CREB in the hippocampus and cortex to 90-95%, BDNF level to 80-90%, restored both synaptophysin and PSD95 in the forebrain. Rescued the spatial learning and memory defects.

Clinical Trial

Molecular Weight

281.39

Formula

C₁₈H₂₆O₃

CAS No.

35380-71-3

SMILES

C[C@@]12[C@](CC[C@@H]2O)([H])[C@@]3([H])[C@@](CC1)([H])C4=CC=C(O)C=C4CC3.O.[1/2]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Elham Hasanzadeh. Defining the role of 17β-estradiol in human endometrial stem cells differentiation into neuron-like cells. Cell Biol Int. 2021 Jan;45(1):140-153. [Content Brief]

[2]. Yujiao Lu, et al. Neuron-Derived Estrogen Regulates Synaptic Plasticity and Memory. J Neurosci. 2019 Apr 10;39(15):2792-2809. [Content Brief]

Estradiol hemihydrate Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Estradiol hemihydrate35380-71-3β-Estradiol hemihydrate17β-Estradiol hemihydrate17β-Oestradiol hemihydrateEstrogen Receptor/ERREndogenous MetaboliteBacterialEstrogenneural differentiationhuman endometrial stem cellsFBN-ARO-KO modelsteroid sex hormoneuterine fibroidsInhibitorinhibitorinhibit

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