2. Academic Validation
  3. Large Peritoneal Macrophages Promote the Resolution of Inflammation in Injured Endometrium

Large Peritoneal Macrophages Promote the Resolution of Inflammation in Injured Endometrium

  • Inflammation. 2025 Aug 2. doi: 10.1007/s10753-025-02335-z.
Jingman Li 1,  Lijie Yin 1,  Jiali Wang 1,  Yuchen Pan 2,  Chen Peng 1,  Yue Dong 1,  Sunan Shen 1 3,  Yayi Hou 1 3,  Guangfeng Zhao 4
Affiliations

Affiliations

  • 1 The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, 210093, China.
  • 2 Jiangsu International Laboratory of Immunity and Metabolism, The Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, China.
  • 3 Jiangsu Key Laboratory of Molecular Medicine, Nanjing, 210093, China.
  • 4 Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210093, China. zhaoguangfeng@nju.edu.cn.
PMID: 40751779 DOI: 10.1007/s10753-025-02335-z
Abstract

Macrophages play a significant role in the repair of endometrial injuries. While large peritoneal macrophages (LPMs) have been reported to migrate to injured organs and repair tissues within the peritoneal cavity, their involvement in the repair of injured endometrium remains unclear. In this study, we utilize a mouse model of endometrial injury that does not involve laparotomy, a procedure that typically results in a substantial loss of LPMs. Strikingly, we find that LPMs reach the endometrium within 6 h post-modeling. By depleting or supplementing LPMs, our results reveal that these cells are capable of engulfing dead cells in the endometrium and resolving inflammation. Additionally, we observe that the migration efficiency of LPMs is enhanced with increased levels of 17β-estradiol (E2) in mice. In vitro assays further confirm that E2 accelerates the migration of LPMs towards apoptotic endometrial stromal cells. Overall, our findings demonstrate that LPMs rapidly migrate into injured endometrium in relation to E2 levels and facilitate the process of tissue repair.

Keywords

17β-estradiol; Endometrial injury; Endometrial repair; Endometrium; Large peritoneal macrophages.

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