Cdk5i peptide 

Cdk5i peptide is a CDK5 inhibitory peptide with high binding affinity for Cdk5/p25 (K_d of 0.17 μM), which can block the interaction between CDK5 and p25 and reduce the kinase activity of CDK5/p25. Cdk5i peptide can be used in the stud y of neurodegenerative diseases^[1].

Cdk5i peptide consists of 12 amino acids (ARAFGIPVRCYS) derived from the CDK5 T loop. This 12-amino-acid sequence is highly conserved in CDK5 across species, yet not present in other Cdk family members^[1].
Cdk5i peptide binds to the Cdk5/p25 complex with more than 92-fold higher affinity than to Cdk5 alone (K_d = 0.17 μM vs. K_d = 15.72 μM)^[1].
Study using co-expressed Cdk5/p25 and Cdk5i peptides in HEK293T cells. Western blot analysis confirmed that coexpression of Cdk5i peptide does not change the amounts of Cdk5 and p25 protein present in cells. However, Cdk5i peptide coexpression does reduce both the interaction of Cdk5 with p25 (~25%) and Cdk5 kinase activity (~27%), while it did not significantly impact the kinase activity of Cdk5/p35 and Cdk1/Cyclin A complexes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

When tagged with a fluorophore (FITC) (HY-66019) and the cell-penetrating transactivator of transcription (TAT) (HY-P0281) sequence, the Cdk5i-FT peptide exhibits cell- and brain-penetrant properties. Cdk5i-FT peptide (20 mg peptide/kg; intraperitoneal injection; for a month) confers protection against neurodegenerative phenotypes associated with Cdk5 hyperactivity in mouse models of neurodegeneration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1339.56

C₆₀H₉₄N₁₈O₁₅S

2298317-00-5

Solid

White to off-white

Ala-Arg-Ala-Phe-Gly-Ile-Pro-Val-Arg-Cys-Tyr-Ser

ARAFGIPVRCYS

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Pao PC, et al. A Cdk5-derived peptide inhibits Cdk5/p25 activity and improves neurodegenerative phenotypes. Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2217864120.  [Content Brief]