Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5)

Cat. No.: HY-P990253: Data Sheet
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Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) is an anti-mouse Delta-like protein 1/DLL1 IgG monoclonal antibody. Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) can reverse the inhibition of Treg cells. Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) can inhibit the upregulation of GLT-1 expression induced by endothelial cells. Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) reduces autoimmune response by reducing B cell activation. Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) can be used for research on inflammation conditions such as lupus nephritis.

For research use only. We do not sell to patients.

Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) Chemical Structure

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Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) Featured Recommendations:

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EAAT1 EAAT2 EAAT3 EAAT4 EAAT5

Biological Activity
Purity & Documentation
References
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Description

Isotype

Armenian hamster IgG

Recommend Isotype Controls

Polyclonal Armenian hamster IgG, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

Delta-like protein 1/DLL1

In Vitro

Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) (20 μg/mL, 24 h) blocks the inhibitory effect of Notch ligand on Treg cells combined with JAG1 mAb and restores the expressions of Foxp3, PD-1, and TGF-β^[1].
Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) (5 µg/mL, 1 h) can significantly inhibit the upregulation of GLT-1 expression induced by endothelial cells^[2].
Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) (20 μg/mL, 3 days) significantly inhibits group 3 innate lymphoid cells (ILC3s) mediated B cell proliferation, differentiation, and antibody production^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Treg cells
Concentration:	20 μg/mL, combined with JAG1 mAb (20 μg/mL)
Incubation Time:	24 h
Result:	Restored the expressions of Foxp3, PD-1, and TGF-β.

Western Blot Analysis^[2]

Cell Line:	bEND.3 cells
Concentration:	5 µg/mL
Incubation Time:	Preprocessing 1 h, co-culture with primary astrocytes 7-10 days.
Result:	Inhibited the level of GLT-1.

In Vivo

Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) (5 mg/kg, i.p., twice a week, for 10 weeks) significantly reduces autoimmune response and kidney damage in female MRL/lpr mice with a spontaneous lupus model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female MRL/lpr mice with a spontaneous lupus model (8 weeks)^[3]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection (i.p.), twice a week, for 10 weeks
Result:	Reduced the number of kidney B cells, plasma cell ratio, and IgG⁺ B cell ratio. Reduced the incidence of skin lesions. Improved the deposition of IgG and C3 in glomeruli. Decreased urinary protein to creatinine ratio.

Gene ID

13388 [NCBI]

Accession

Q61483

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

SMILES

[Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Rong H, et al. Notch signalling suppresses regulatory T-cell function in murine experimental autoimmune uveitis. Immunology. 2016 Dec;149(4):447-459. [Content Brief]

[2]. Martinez-Lozada Z, et al. Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression. Neurochem Int. 2020 Oct;139:104787. [Content Brief]

[3]. Li F, et al. Group 3 Innate Lymphoid Cells Exacerbate Lupus Nephritis by Promoting B Cell Activation in Kidney Ectopic Lymphoid Structures. Adv Sci (Weinh). 2023 Dec;10(35):e2302804. [Content Brief]

Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5) Related Classifications

Inflammation/Immunology

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse Delta-like protein 1/DLL1 Antibody (HMD1-5)NotchEAATExcitatory amino acid transporter Glutamate transporter| Delta-like protein 1/DLL1TregGLT-1B celllupus nephritisInhibitorinhibitorinhibit

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