2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Proliferation and Cytotoxicity
  6. Cell Counting Kit-8

Cell Counting Kit-8 

Cat. No.: HY-K0301
Manual COA SDS Technical Support

Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.

Cell Counting Kit-8
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2894 Publications Citing Use of MCE Cell Counting Kit-8

Cell Viability Assay
MTT assay
Proliferation Assay

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Feb 7;9(2):177.  [Abstract]

    CCK8 assay. Proliferation of CAR T cells during expansion in response to repetitive stimulation with EGFR antigen.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2018 Apr;15(4):4129-4136.  [Abstract]

    The effect of TRPM8 knockdown on (A) LNCaP and (B) PC3 cells proliferation is measured by CCK 8 assay.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: J Mol Liq. 2023 Mar 2.

    CCK-8 (10 μM; 24 h) is used to detect the survival rate of L929 cells after treatment with different concentrations of DESs.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Mar 8;9(1):63.  [Abstract]

    CCK8 (1-2 h) assay-based detection of IC50 of gemcitabine in ICC cells.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2022 Jul 14;20(1):324.  [Abstract]

    CCK-8 (10 μM; 1-2 h) assay was used to assess the effect of huc-MSCs-derived exosomes (1, 10, 50, 100 µg/ml) on BV2 cell viability, and ns indicates no significance.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 13:e2413728.  [Abstract]

    CCK-8 (2 h). Absorption at 450 nm in MTT assay of different incubation groups after12, 24, and 48 hours of incubation.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Nov 26;18(47):32578-32588.  [Abstract]

    CCK-8 staining with ECs and ASMCs.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Oct 16.  [Abstract]

    CCK-8 (10 μL/well; 50 min). (B,C) Quantitative determination of neuroprotection in the SH-SY5Y cell model for neuronal damage induced by Aβ1−42 oligomers.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Sep 25.  [Abstract]

    CCK-8 (10 μL/well; incubated at 37°C for 1-4 hours). CCK-8 assay is used to measure cell counts of PC9 Del19 lung cancer cells and normal cells NHBE (normal bronchial epithelial cells), 16HBE (normal bronchial epithelial cells), BEAS-2B (normal bronchial epithelial cells), MCF10A (normal breast epithelial cells), IMR-90 (lung fibroblasts), primary B cells (lymphocytes), and primary CD8+ T cells (lymphocytes).

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Ann Rheum Dis. 2024 Sep 4:ard-2024-225565.  [Abstract]

    CCK-8 (10 µL/well). Viability of RA-FLSs after daily treatment with phosphate buffered saline (PBS) or 400 nM negative control (NC), SAPT4 or SAPT8 for 5 days as determined by Cell Counting Kit-8 assays.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Apr 12;S1550-4131(23)00095-5.  [Abstract]

    CCK-8 (10 μL/well; 2 h). CCK-8 assays were carried out to assess the effect of TK1 on HCC cell proliferation. n = 3 independent biological replicates.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2024 Jul 16.

    CCK-8 (10 μL/well; 2 h). The cytotoxicity of OMV-CD47/GPC3 on Hepa1-6, HK-2, and L-02 cells.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Nano Energy. 2024 Sep.

    CCK-8 (10 μL/well; 2 h). Cell viability of L929 cultured in the serum-supplemented MEM basic containing UPN extract or not after 1, 2 and, 3 days (n = 3).

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2023 Oct 23:gkad900.  [Abstract]

    CCK-8 (10 μL/well; 1 h). Cell Counting Kit-8 (CCK-8) cytotoxicity assays on (A) HEK293T cells, (B) HGC-27 cells and (C) HeLa cells. Cells were incubated with 0-1000 nM of L-Apt12-6 for 48 h, and then were incubated with CCK-8 for 1 h to evaluate cytotoxicity.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: Drug Resist Updat. 2024 Mar:73:101052.

    CCK-8 (10 μL/well; 1-4 h). HCC cells, divided into DUSP4-vehicle and DUSP4-OE (overexpression) groups, were exposed to different concentrations of sorafenib (0, 2.5, 5, 10, 15, and 20 µM) for 24 h, followed by assessment of cell viability via the CCK8 assay.

    Cell Counting Kit-8 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Jul;13(7):e12473.  [Abstract]

    CCK-8 (10 μL/well; 1 h). The HUVECs proliferation in each group determined by CCK‐8 assay (n = 3/group).

    MCE Biological Validation

    Proliferation Assay
    MTT assay
    Cell Viability Assay

      Cell Counting Kit-8 purchased from MCE. Usage Cited in: Cell Metab. 2023 Apr 12;S1550-4131(23)00095-5.  [Abstract]

      Customer Validation
      CCK-8 (10 μL/well; 2 h). CCK-8 assays were carried out to assess the effect of TK1 on HCC cell proliferation. n = 3 independent biological replicates.

      Cell Counting Kit-8 purchased from MCE. Usage Cited in: Adv Mater. 2025 Jan 13:e2413728.  [Abstract]

      Customer Validation
      CCK-8 (2 h). Absorption at 450 nm in MTT assay of different incubation groups after12, 24, and 48 hours of incubation.

      Cell Counting Kit-8 purchased from MCE. Usage Cited in: Ann Rheum Dis. 2024 Sep 4:ard-2024-225565.  [Abstract]

      Customer Validation
      CCK-8 (10 µL/well). Viability of RA-FLSs after daily treatment with phosphate buffered saline (PBS) or 400 nM negative control (NC), SAPT4 or SAPT8 for 5 days as determined by Cell Counting Kit-8 assays.

      Cell Counting Kit-8 purchased from MCE. Usage Cited in: Adv Funct Mater. 2024 Jul 16.

      Customer Validation
      CCK-8 (10 μL/well; 2 h). The cytotoxicity of OMV-CD47/GPC3 on Hepa1-6, HK-2, and L-02 cells.

      • Description

      • Storage

      • Application

      • Attention

      • Components

      • Documentation

      Description
      & Advantages

      Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.
      Storage

      4°C, 2 years

      Keep away from light for long-term storage.
      Application

      1. Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the CCK-8 assay.

      2. WST-8 may react with reducing agents to generate WST-8 formazan. Please check the background O.D. if reducing agents are used in cytotoxicity assays or cell proliferation assays.

      3. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

      4. If the sterilization of the CCK-8 solution is necessary, please filter the solution with a 0.2 μm membrane.

      5. The incubation time varies by the type and number of cells in a well. Generally, leukocytes give weak coloration, thus a long incubation time (up to 4 hours) or a large number of cells (~105 cells/well) may be necessary.

      6. Measure and subtract the O.D. at 600 nm or higher from that of sample if there is a high turbidity in the cell suspension.

      7. This product is for R&D use only, not for drug, household, or other uses.

      8. For your safety and health, please wear a lab coat and disposable gloves to operate.
      Attention

      1. Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the CCK-8 assay.

      2. WST-8 may react with reducing agents to generate WST-8 formazan. Please check the background O.D. if reducing agents are used in cytotoxicity assays or cell proliferation assays.

      3. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

      4. If the sterilization of the CCK-8 solution is necessary, please filter the solution with a 0.2 μm membrane.

      5. The incubation time varies by the type and number of cells in a well. Generally, leukocytes give weak coloration, thus a long incubation time (up to 4 hours) or a large number of cells (~105 cells/well) may be necessary.

      6. Measure and subtract the O.D. at 600 nm or higher from that of sample if there is a high turbidity in the cell suspension.

      7. This product is for R&D use only, not for drug, household, or other uses.

      8. For your safety and health, please wear a lab coat and disposable gloves to operate.
      Components
      Cat.No. Product Name Package
      HY-K0301-1 mL Cell Counting Kit-8 1 mL
      HY-K0301-5 mL Cell Counting Kit-8 5 mL
      HY-K0301-30 mL Cell Counting Kit-8 5 mL × 6
      HY-K0301-120 mL Cell Counting Kit-8 5 mL × 24
      Documentation

      Manual (446 KB) SDS (159 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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