M2 microglia-derived exosomes reduce neuronal ferroptosis via FUNDC1-mediated mitophagy by activating AMPK/ULK1 signaling

Neuronal Ferroptosis plays a vital role in the progression of neonatal hypoxic-ischemic brain damage (HIBD). M2-type microglia-derived exosomes (M2-exos) have been shown to protect neurons from ischemia-reperfusion (I/R) brain injury, but their impact on I/R-induced neuronal Ferroptosis and the underlying mechanisms remain poorly understood. In this study, we used an in vitro oxygen-glucose deprivation/reoxygenation (OGD/R) model in HT-22 neuronal cells to investigate how M2-exos modulate Ferroptosis. We found that M2-exos were internalized by HT-22 cells and significantly attenuated OGD/R-induced Ferroptosis. Mechanistically, M2-exos enhanced Mitophagy, which was mediated by the upregulation of FUN14 domain-containing protein 1 (FUNDC1), thereby inhibiting Ferroptosis. Further analysis revealed that M2-exos activated FUNDC1-dependent Mitophagy through the AMP-activated protein kinase (AMPK)/UNC-51-like kinase 1 (ULK1) signaling pathway. Taken together, these findings suggest that M2-exos ameliorate I/R-induced neuronal Ferroptosis by enhancing FUNDC1-mediated Mitophagy through the activation of AMPK/ULK1 signaling pathway.

Exosome; Ferroptosis; Ischemia/reperfusion; Microglia; Mitophagy.