2. Protein Tyrosine Kinase/RTK PI3K/Akt/mTOR Stem Cell/Wnt MAPK/ERK Pathway Metabolic Enzyme/Protease
  3. Src Akt ERK TAM Receptor c-Met/HGFR MMP HIF/HIF Prolyl-Hydroxylase VEGFR
  4. AG01

AG01 is a monoclonal antibody against progranulin (GP88). AG01 inhibits triple-negative breast cancer (TNBC) cell proliferation and migration, reduces the expression of phosphorylated protein kinases p-Src, p-AKT, and p-ERK, and reduces the expression of oncogenic proteins such as Axl, c-MET, HIF-1α, and VEGF. AG01 inhibits tumor growth and Ki67 expression in a TNBC xenograft mouse model. AG01 can be used in the research of TNBC and other cancers.

For research use only. We do not sell to patients.

AG01

AG01 Chemical Structure

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AG01 Related Antibodies

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Axl Mer Tyro3

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VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4

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Description

AG01 is a monoclonal antibody against progranulin (GP88). AG01 inhibits triple-negative breast cancer (TNBC) cell proliferation and migration, reduces the expression of phosphorylated protein kinases p-Src, p-AKT, and p-ERK, and reduces the expression of oncogenic proteins such as Axl, c-MET, HIF-1α, and VEGF. AG01 inhibits tumor growth and Ki67 expression in a TNBC xenograft mouse model. AG01 can be used in the research of TNBC and other cancers[1].

Species Reactivity

Human
IC50 & Target[1]

ERK1

 

ERK2

 

MMP-2

 

Axl

 

HIF-1α

 

In Vitro

AG01 (0-300 µg/mL, 48-72 h) inhibits cells proliferation, reduces Ki67-positive nuclei quantification in MDA-MB-231, HS578-T cells[1].
AG01 (0-100 µg/mL, 5 h) inhibits cells migration and invasion in MDA-MB-231, HS578-T cells[1].
AG01 (100 µg/mL, 24-72 h) decreases the phosphorylation status of AKt, Src, Erk1/2 in MDA-MB-231, HS578-T cells[1].
AG01 (100 µg/mL, 24-72 h) decreases the proteins expression of Axl, c-MET, ICAM-1, MMP-2, SNAIL, HIF-1α, VEGF, endogenous progranulin, SPARC, cell adhesion and migratory protein-fbronectin in MDA-MB-231, HS578-T cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AG01 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MDA-MB-231, HS578-T cells
Concentration: 0, 25, 50, 100, 200, 300 µg/mL
Incubation Time: 48, 72 h
Result: Inhibited cells proliferation, resulted in a dose-dependent inhibition of MDA-MB-231 and HS578-T cells proliferation by 44% and 51% at 300 µg/mL compared with control.

Cell Migration Assay [1]

Cell Line: MDA-MB-231, HS578-T cells
Concentration: 25, 50, 100 µg/mL
Incubation Time: 5 h
Result: Inhibited cells migration in a dose-dependent, with the highest reduction of 78.2%, 77.5% at 100 µg/mL for MDA-MB-231, HS578-T cells.

Western Blot Analysis[1]

Cell Line: MDA-MB-231, HS578-T cells
Concentration: 100 µg/mL
Incubation Time: 24, 48, 72 h
Result: Decreased the phosphorylation status of Akt (14.8%), Src (23.6%), and Erk1/2 (20.3%) at 24 h and decreased phosphorylation of Akt (63%), Src (55.4%), and Erk1/2 (55.2%) at 72 h compared to control, decreased the ratio of phosphorylated protein to its total corresponding protein expression for Akt (71.9%), Src (49.8%), and Erk1/2 (62%) at 72 h in MDA-MB-231 cells.
Decreased the phosphorylation status of Akt (44.5%), Src (31.5%), and ERK1/2 (35.5%) at 24 h while decreased FAK phosphorylation (44.5%) at 48 h, decreased the ratio of phosphorylated protein to its total corresponding protein expression for p-Src/Src (44.3%), p-Akt/Akt (47.3%), and p-ERK1/2/ERK (46%) at 48 h in HS578-T cells.
Reduced c-MET expression of 59.7%.
Reduced SNAIL expression of 23.3% and 89%, ICAM-1 of 93.8% and 33.8%, AXL of 13.2% and 33.4% in MDA-MB-231 and HS578-T cells, respectively, reduced endogenous progranulin level in time-dependent reduction with MDA-MB-231 showing 37.7% reduction by 72 h and HS578-T showing almost a 100% reduction by 48 h.
Reduced cell adhesion and migratory protein-fbronectin in time-dependent decrease of 25.4% at 72 h as compared to its control lysate in MDA-MB-231 cells.
In Vivo

AG01 (10 mg/kg, i.p., twice weekly, 40 days) inhibits tumor growth and Ki67 expression in MDA-MB-231 xenograft nude mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MDA-MB-231 (1.5x106) xenograft nude mice (6-8-week-old, female) model[1]
Dosage: 10 mg/kg
Administration: i.p., twice weekly, 40 days
Result: Reduced Vt/V0 ratio and tumor weights by 50% compared to control human IgG treated group, reduced Ki67 expression in tumor sections by 52.5% compared to control groups, showed a 50% reduction of the mitotic index compared to control and 65% reduction of relative microvessel numbers compared to control HuIgG-treated animals.
Gene ID

2896  [NCBI]

Accession

NP_002078.1
Target

PGRN/GP88
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

N/A
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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AG01 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AG01AG 01AG-01SrcAktERKTAM Receptorc-Met/HGFRMMPHIF/HIF Prolyl-HydroxylaseVEGFRPKBProtein kinase BExtracellular signal regulated kinasesTyro3AxlMerMatrix metalloproteinasesHypoxia-inducible factorsHIFsHIF-PHVascular endothelial growth factor receptorAntibodyMDA-MB-231 cellsHS578-T cellsTNBCnude miceInhibitorinhibitorinhibit

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