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  4. SPSB4 Protein, Human (His)

SPSB4 Protein, Human (His) is the recombinant human-derived SPSB4, expressed by E. coli , with His labeled tag. ,

For research use only. We do not sell to patients.

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Description

SPSB4 Protein, Human (His) is the recombinant human-derived SPSB4, expressed by E. coli , with His labeled tag. ,
Background

SPSB4 is the substrate recognition component of an SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins (by similar). It negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (by similar). SPSB4 acts as a bridge linking NOS2 with the ECS E3 ligase complex components ELOC and CUL5 (by similar). It diminishes EphB2-dependent cell repulsive responses by mediating the ubiquitination and degradation of EphB2/CTF2 (by similar), and regulates cellular clock function by mediating the ubiquitin/proteasome-dependent degradation of the circadian transcriptional repressor NR1D1.
Species

Human
Source

E. coli
Tag

His
Accession

Q96A44 (R33-E233)
Gene ID

92369
Protein Length

Partial
Synonyms
SSB4
Predicted Molecular Mass
23.7 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SPSB4 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SPSB4 Protein, Human (His)
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