SPOP Protein, Human

SPOP Protein, Human is the recombinant human-derived SPOP, expressed by E. coli , with tag Free labeled tag. ,

SPOP is a component of the cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates target protein ubiquitination, often leading to proteasomal degradation. In complex with CUL3, it ubiquitinates and degrades BRMS1, DAXX, PDX1/IPF1, GLI2, and GLI3, while ubiquitinating MACROH2A1 and BMI1 without degradation. It suppresses PDX1/IPF1 target gene (e.g., insulin) activation by promoting PDX1/IPF1 degradation. The homodimeric SPOP-containing BCR complex exhibits higher ubiquitin ligase activity than the SPOP/SPOPL heterodimer-containing complex. It regulates BET proteins (BRD2/3/4) stability, controls translation (not degradation) of DNA replication factors CDT1 and CDC6 for DNA synthesis, targets IRF1 via S/T-rich degrons, restricts EV71 replication by lysosomal degradation of viral protease 2A, and inhibits HBV via HNF1A ubiquitination. Additionally (by similar), it ubiquitinates BRDT to regulate histone removal during spermatogenesis.

Human

E. coli

Tag Free

O43791 (K28-V168)

8405

Partial

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.