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  3. SPEB Protein, Streptococcus pyogenes (His-SUMO)

SPEB Protein, Streptococcus pyogenes (His-SUMO)

Cat. No.: HY-P704245
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products
  • Technical Parameters

  • Properties

Species

Others

Source

E. coli

Tag

N-6*His;N-SUMO

Accession

P0C0J0 (Q146-P398)

Gene ID

69901512

Molecular Construction
N-term
6*His
SUMO
SPEB (Q146-P398)
Accession # P0C0J0
C-term
Protein Length

Full Length of Mature Protein

Synonyms
speB; Streptopain; EC 3.4.22.10; Exotoxin type B; SPE B; Streptococcal cysteine proteinase; Streptococcus peptidase A; SPP
AA Sequence

QPVVKSLLDSKGIHYNQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTLSSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDYGPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGVGKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSAVVGIKP

Predicted Molecular Mass
43.6kDa
Molecular Weight

Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

Structure/Form
Full Length of Mature Protein
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

SPEB Protein, Streptococcus pyogenes (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SPEB Protein, Streptococcus pyogenes (His-SUMO)
Cat. No.:
HY-P704245
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