Siglec-9 Protein, Cynomolgus (HEK293, His)

Siglec-9 Protein is a member of the Siglec cell surface immunoglobulin family. Siglec-9 is highly expressed on human neutrophils and monocytes and low on natural killer cells, and sub-populations of T and B lymphocytes. Siglec-9 induces human neutrophil apoptosis and autophagy-like cell death and also inhibits tumor activity^[1][2][3]. Siglec-9 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-9 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-9 Protein, Cynomolgus (HEK293, His) is 331 a.a., with molecular weight of 55-70 kDa.

Sialic acid-binding immunoglobulin-like lectin (Siglec-9) is a member of the Siglec cell surface immunoglobulin family. Siglec-9 is highly expressed on human neutrophils and monocytes and low on natural killer cells, and sub-populations of T and B lymphocytes. Ligation of Siglec-9 by chemical compounds or synthetic ligands induced apoptosis and autophagic-like cell death in human neutrophils. Siglec-9 binds to sialic acid and transduces apoptotic and nonapoptotic death signals to neutrophils. Siglec-9 is an immune-checkpoint molecule on macrophages that can be targeted to enhance anti-PD-1/PD-L1 therapeutic efficacy for GBM treatment. Pro-tumorigenic activity can be suppressed by blocking Siglec-9, which unleashes antitumor functions and induces robust tumor-eliminating activity. Targeting Siglec-9 could be beneficial for the treatment of COPD, asthma, fibrosis, and related chronic inflammatory lung diseases^[1][2][3].

Immobilized Cynomolgus Siglec-9 His at 10 μg/mL (100 μL/well) on Nickel Coated plate can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 10-156 ng/mL.

Cynomolgus

HEK293

C-His

XP_005590140 (Q20-G350)

102118310  [NCBI]

55-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of 25 mM MES, 150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.