1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. C1-Inhibitor
  6. Serpin G1 Protein, Human (500a.a, HEK293, His)

Serpin G1 Protein, Human (500a.a, HEK293, His)

Cat. No.: HY-P73526
COA Handling Instructions

Serpin G1, also known as C1-inhibitor, controls C1 complex activation by forming a proteolytically inactive complex with C1r or C1s proteases. Its interactions regulate physiological pathways like complement activation, blood coagulation, fibrinolysis, and kinin generation. As a potent inhibitor of FXIIa, chymotrypsin, and kallikrein, Serpin G1 binds to E. coli stcE, protecting against complement-mediated lysis, and engages with MASP1, showcasing its intricate involvement in various cellular processes. Serpin G1 Protein, Human (500a.a, HEK293, His) is the recombinant human-derived Serpin G1 protein, expressed by HEK293, with C-10*His labeled tag. The total length of Serpin G1 Protein, Human (500a.a, HEK293, His) is 478 a.a., with molecular weight of ~110 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $136 In-stock
10 μg $230 In-stock
100 μg $1100 Get quote
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  • Biological Activity

  • Technical Parameters

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Description

Serpin G1, also known as C1-inhibitor, controls C1 complex activation by forming a proteolytically inactive complex with C1r or C1s proteases. Its interactions regulate physiological pathways like complement activation, blood coagulation, fibrinolysis, and kinin generation. As a potent inhibitor of FXIIa, chymotrypsin, and kallikrein, Serpin G1 binds to E. coli stcE, protecting against complement-mediated lysis, and engages with MASP1, showcasing its intricate involvement in various cellular processes. Serpin G1 Protein, Human (500a.a, HEK293, His) is the recombinant human-derived Serpin G1 protein, expressed by HEK293, with C-10*His labeled tag. The total length of Serpin G1 Protein, Human (500a.a, HEK293, His) is 478 a.a., with molecular weight of ~110 kDa.

Background

Serpin G1, known as C1-inhibitor, exerts control over the activation of the C1 complex by forming a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. This interaction highlights its potential pivotal role in regulating essential physiological pathways, including complement activation, blood coagulation, fibrinolysis, and kinin generation. Functioning as a highly efficient inhibitor of FXIIa, it also inhibits chymotrypsin and kallikrein. Remarkably, Serpin G1 binds to E. coli stcE, facilitating its localization to cell membranes and thereby safeguarding the bacteria against complement-mediated lysis. Additionally, it engages with MASP1, further contributing to its intricate involvement in diverse cellular processes.

Biological Activity

Measured by its ability to inhibit Recombinant Human Complement Component C1s cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC50 is 10.393 nM, as measured under the described conditions.

Species

Human

Source

HEK293

Tag

C-10*His

Accession

P05155/NP_000053.2 (N23-A500)

Gene ID

710  [NCBI]

Molecular Construction
N-term
Serpin G1 (N23-A500)
Accession # P05155/NP_000053.2
10*His
C-term
Synonyms
C1IN; C1INH; SERPING1; Serpin G1; Plasma protease C1 inhibitor
AA Sequence

NPNATSSSSQDPESLQDRGEGKVATTVISKMLFVEPILEVSSLPTTNSTTNSATKITANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGSFCPGPVTLCSDLESHSTEAVLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPRA

Molecular Weight

Approximately 110 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Serpin G1 Protein, Human (500a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Serpin G1 Protein, Human (500a.a, HEK293, His)
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