2. Recombinant Proteins
  3. Viral Proteins
  4. SARS-CoV-2 Proteins
  5. SARS-CoV-2 3C-like Protease
  6. SARS-CoV-2 3CLpro/3C-like protease Protein

SARS-CoV-2 3CLpro/3C-like protease Protein is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by E. coli, with tag free.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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  • Biological Activity

  • Technical Parameters

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Description

SARS-CoV-2 3CLpro/3C-like protease Protein is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by E. coli, with tag free.
Biological Activity

Measured by its ability to cleave a fluorogenic peptide substrate Dabcyl-KTSAVLQSGFRKME-Edans and the specific activity is ≥1068.3 nmol/min/mg.
Species

Virus
Source

E. coli
Tag

Tag Free
Accession

YP_009725301.1 (S1-Q306)
Gene ID

43740578  [NCBI]
Molecular Construction
N-term
SARS-CoV-2 3CLpro (S1-Q306)
Accession # YP_009725301.1
C-term
Protein Length

Full Length
Synonyms
3C-like protease; 3CL Protease; 3CL-Mpro; 3CL Pro; COVID-19; M Proteinase; Mpro
Molecular Weight

Approximately 33.8 kDa
Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Solution
Formulation

Supplied as a 0.22 μm filtered solution of 25 mM HEPES, 2.5 mM DTT, 10% glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.
Documentation
SDS (251 KB)

COA (205 KB)

Handling Instructions (2659 KB)

SARS-CoV-2 3CLpro/3C-like protease Protein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SARS-CoV-2 3CLpro/3C-like protease Protein
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