RNF43 Protein, Human (HEK293, Fc)

RNF43 is an E3 ubiquitin protein ligase and a key negative regulator in the Wnt signaling pathway. Its role involves mediating the ubiquitination, endocytosis, and subsequent degradation of components within the Wnt receptor complex, particularly Frizzled. RNF43 Protein, Human (HEK293, Fc) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag.

RNF43, an E3 ubiquitin-protein ligase, serves as a critical negative regulator of the Wnt signaling pathway by orchestrating the ubiquitination, endocytosis, and subsequent degradation of key components in the Wnt receptor complex, specifically targeting Frizzled. This regulatory activity extends to both canonical and non-canonical Wnt signaling pathways. RNF43's role is underscored by its involvement as part of a master switch, alongside RSPO2 and ZNRF3, governing limb specification. This implies its significance in developmental processes and highlights its multifaceted involvement in modulating Wnt signaling, making it a crucial player in cellular and developmental pathways.

Immobilized Human RSPO3 His at 2 μg/mL (100 μL/well) can bind Human RNF43 hFc, the EC₅₀ of Human RNF43 hFc is 3.0-12.0 ng/mL.

Human

HEK293

C-hFc

Q68DV7-1 (G24-Y197)

54894  [NCBI]

Extracellular Domain

GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY

Approximately 52 kDa,based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.