1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. RAB27A Protein, Human (His)

RAB27A Protein, Human (His) is the recombinant human-derived RAB27A, expressed by E. coli , with His labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
10 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μg   Get quote  
Synthetic products have potential research and development risk.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

RAB27A Protein, Human (His) is the recombinant human-derived RAB27A, expressed by E. coli , with His labeled tag. ,

Background

Rab27A is a Small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active form, it binds to various effector proteins to regulate homeostasis of the late endocytic pathway, including endosomal positioning, maturation, and secretion. It plays a role in cytotoxic granule exocytosis in lymphocytes and is essential for both granule maturation and granule docking and priming at the immunologic synapse.

Species

Human

Source

E. coli

Tag

His

Accession

P51159-1 (S2-S192, Q78L, C123S, C188S)

Gene ID

5873

Protein Length

Partial

Synonyms
RAB27
Predicted Molecular Mass
23.7 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

RAB27A Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
RAB27A Protein, Human (His)
Cat. No.:
HY-P703183
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