Protein S/PROS1 Protein, Human (396a.a, HEK293, His)

Protein S (PS) is a vitamin K-dependent glycoprotein which plays an important role in the regulation of blood coagulation. Protein S/PROS1 Protein, Human (396a.a, HEK293, His) is the recombinant human-derived protein S/PROS1 protein, expressed by HEK293 , with C-His labeled tag.

Protein S (PS) is a vitamin K-dependent glycoprotein that acts as a natural anticoagulant. It is synthesized by hepatocytes, endothelial cells, megakaryocytes, testis Leydig cells and in brain. Protein S and APC form the activated protein C (APC) complex, which is responsible for the proteolytic deactivation of activated factors (F) V (Va) and VIII (VIIIa). Protein S is also identified as a cofactor of tissue factor pathway inhibitor (TFPI), which stimulates inhibition of the FXa by TFPI through the APC-independent mechanism^[1].

Human

HEK293

C-His

P07225 (V284-S676)

5627

Approximately 53-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wypasek E, et al. Venous thromboembolism associated with protein S deficiency due to Arg451* mutation in PROS1 gene: a case report and a literature review. J Genet. 2017 Dec;96(6):1047-1051.  [Content Brief]