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  4. Plectin Protein, Human (HEK293, His)

Plectin Protein, Human (HEK293, His) is the recombinant human-derived Plectin protein, expressed by HEK293, with N-His tag.

For research use only. We do not sell to patients.

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Description

Plectin Protein, Human (HEK293, His) is the recombinant human-derived Plectin protein, expressed by HEK293, with N-His tag.
Background

Plectin is a versatile cytoskeletal protein that interlinks intermediate filaments with microtubules and microfilaments, and anchors intermediate filaments to desmosomes or hemidesmosomes. It can also bind muscle proteins such as actin to membrane complexes, potentially playing a role in muscle function. Plectin may be involved not only in the filaments network organization but also in regulating their dynamics. As a structural component of muscle, its isoform 9 plays a major role in maintaining myofiber integrity.
Species

Human
Source

HEK293
Tag

N-His
Accession

Q15149-1 (A4377-A4684)
Gene ID

5339
Molecular Construction
N-term
His
PLEC (A4377-A4684)
Accession # Q15149-1
C-term
Synonyms
Plectin; PLEC; Hemidesmosomal protein 1 (HD1); PLEC1; PCN; PLTN
Predicted Molecular Mass
34.5 kDa
Molecular Weight

Approximately 43-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Plectin Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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