1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PAD6 Protein, Human (sf9, His)

PAD6 Protein, Human (sf9, His) is the recombinant human-derived PAD6 protein, expressed by sf9 insect cells, with N-His tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
50 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μg   Get quote  
Synthetic products have potential research and development risk.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PAD6 Protein, Human (sf9, His) is the recombinant human-derived PAD6 protein, expressed by sf9 insect cells, with N-His tag.

Background

PAD6 is a structural constituent of cytoplasmic lattices, playing a key role in early embryonic development. Cytoplasmic lattices are fibrous structures found in the cytoplasm of oocytes and preimplantation embryos, essential for storing maternal proteins critical for embryonic development, such as ribosomal proteins and proteins involved in epigenetic reprogramming of the preimplantation embryo, while preventing their degradation or activation. Unlike other family members, PAD6 lacks protein-arginine deiminase activity due to its inability to bind Ca2+. by similar: Function and composition of cytoplasmic lattices.

Species

Human

Source

sf9 insect cells

Tag

N-His

Accession

Q6TGC4 (M1-P694)

Gene ID

353238

Molecular Construction
N-term
His
PADI6 (M1-P694)
Accession # Q6TGC4
C-term
Synonyms
PADI6; PREMBL2; Peptidylarginine Deiminase VI; Peptidylarginine Deiminase Type 6
Predicted Molecular Mass
79.7 kDa.
Molecular Weight

Approximately 80 kDa, based on SDS-PAGE under reducing conditions.

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH7.5 with glycerol as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

PAD6 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PAD6 Protein, Human (sf9, His)
Cat. No.:
HY-P704835
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