Neuropoietin Protein, Mouse

Neurogenin, a protein associated with elevated platelet counts and splenomegaly, is involved in neuronal precursor development and maturation. By binding to the CNTF receptor complex (including CNTF alpha chain, LIFR and IL6ST), neurogenin may affect signaling pathways related to cell differentiation. Neuropoietin Protein, Mouse is the recombinant mouse-derived Neuropoietin protein, expressed by E. coli , with tag free.

Neuropoietin, a protein associated with increased platelet count and splenomegaly, potentially plays a crucial role in neuronal precursor development and maturation. It exerts its effects by binding to the tripartite CNTF receptor complex, which comprises the CNTF alpha chain, LIFR, and IL6ST. This interaction, observed in vitro, suggests Neuropoietin's involvement in signaling pathways associated with cellular differentiation and maturation processes. The protein's dual roles in platelet regulation and neurodevelopment underscore its versatility and potential significance in diverse physiological contexts.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 50-200 ng/mL.

Mouse

E. coli

Tag Free

P83714 (A23-A204)

244218  [NCBI]

Full Length of Mature Protein

Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.