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  4. MAT1A Protein, Human (His)

MAT1A Protein, Human (His) is the recombinant human-derived MAT1A protein, expressed by E. coli, with N-10*His tag.

For research use only. We do not sell to patients.

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Description

MAT1A Protein, Human (His) is the recombinant human-derived MAT1A protein, expressed by E. coli, with N-10*His tag.
Background

MAT1A is an enzyme that catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The reaction involves two steps, both catalyzed by the same enzyme: the formation of S-adenosylmethionine (AdoMet) and triphosphate, followed by the hydrolysis of the triphosphate.
Species

Human
Source

E. coli
Tag

N-10*His
Accession

Q00266 (M1-F395)
Gene ID

4143
Molecular Construction
N-term
10*His
MAT1A (M1-F395)
Accession # Q00266
C-term
Protein Length

Full Length
Synonyms
AdoMet synthase 1; Methionine adenosyltransferase 1(MAT 1); Methionine adenosyltransferase I/III(MAT-I/III)
AA Sequence

MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF

Predicted Molecular Mass
47.3 kDa
Molecular Weight

Approximately 45-50 kDa, based on SDS-PAGE under reducing conditions.
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MAT1A Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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