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  2. Biotinylated Proteins
  3. LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi)

LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78837
Handling Instructions Technical Support

LRP-6 protein is a key component of the Wnt-Fzd-LRP5-LRP6 complex, a component of β-catenin signaling, and is essential for bone formation. LRP-6 acts as a cell surface co-receptor and coordinates the formation of signalosomes by clustering receptor-ligand complexes. LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRP-6 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi) is 611 a.a., with molecular weight of 80-100 kDa.

For research use only. We do not sell to patients.

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25 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
200 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
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  • Biological Activity

  • Technical Parameters

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Description

LRP-6 protein is a key component of the Wnt-Fzd-LRP5-LRP6 complex, a component of β-catenin signaling, and is essential for bone formation. LRP-6 acts as a cell surface co-receptor and coordinates the formation of signalosomes by clustering receptor-ligand complexes. LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRP-6 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi) is 611 a.a., with molecular weight of 80-100 kDa.

Background

As an integral component of the Wnt-Fzd-LRP5-LRP6 complex, LRP-6 protein plays a crucial role in initiating beta-catenin signaling by orchestrating the aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Functioning as a cell-surface coreceptor in Wnt/beta-catenin signaling, it is particularly pivotal in bone formation. The complex formed by Wnt-induced Fzd/LRP6 coreceptor recruits DVL1 polymers to the plasma membrane, facilitating the recruitment of the AXIN1/GSK3B complex to the cell surface. This, in turn, promotes the formation of signalosomes, inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. LRP-6 is indispensable for the posterior patterning of the epiblast during gastrulation. The protein engages in a myriad of interactions, including those with various WNT proteins, FZD5, DKK1, DRAXIN, AXIN1, GRB10, RSPO1, RSPO3, SOST, MESD, CSNKIE, MACF1, DAB2, TMEM198, CAPRIN2, LYPD6, KREM1, and MDK, highlighting its versatility and regulatory roles in the Wnt signaling pathway.

Biological Activity

Immobilized Human R-Spondin 1 His at 1 μg/mL (100 μL/well) can bind Biotinylated Human LRP-6 His-Avi with a linear range of 20-156 ng/mL.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

O75581  (A20-P630)

Gene ID
Synonyms
LRP-6; LRP6
Molecular Weight

80-100 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
LRP-6 Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P78837
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