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  5. LAMP1/CD107a Protein, Human (HEK293, Fc)

LAMP1/CD107a Protein, Human (HEK293, Fc)

Cat. No.: HY-P7779
COA Handling Instructions

LAMP1/CD107a Protein, Human (HEK293, Fc) is a recombinant human CD107a expressed in HEK 293 cells with a C-Fc tag at the C-terminus. LAMP1/CD107a Protein can be used as a functional marker for the identification of natural killer cell activity.

For research use only. We do not sell to patients.

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10 μg $105 In-stock
50 μg $314 In-stock
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Description

LAMP1/CD107a Protein, Human (HEK293, Fc) is a recombinant human CD107a expressed in HEK 293 cells with a C-Fc tag at the C-terminus. LAMP1/CD107a Protein can be used as a functional marker for the identification of natural killer cell activity[1][2].

Background

Lysosomal associated membrane protein 1 (LAMP1 or CD107a) is a highly glycosylated lysosomal and endosomal membrane protein of 120 kDa. LAMPs and LIMPs are tightly packed and represent more than 50% of the total membrane protein of late endosomes and lysosomes. LAMP1 has been described as a marker of CD8+ T-cell degranulation following stimulation. LAMP1 can be used as a functional marker for the identification of natural killer cell activity[1][2].

Biological Activity

Immobilized Human LGALS3 at 2μg/ml (100 μl/well) can bind recombinant Human CD107a, C-Fc (HEK293-expressed) (rHuCD107a, C-Fc). The ED50 of recombinant Human CD107a, C-Fc (HEK293-expressed) (rHuCD107a, C-Fc) is 1-5 ug/mL.

Species

Human

Source

HEK293

Tag

C-Fc

Accession

P11279-1 (A29-M382)

Gene ID
Molecular Construction
N-term
LAMP1 (A29-M382)
Accession # P11279-1
Fc
C-term
Synonyms
rHuCD107a, C-Fc; Lysosome-Associated Membrane Glycoprotein 1; LAMP-1; Lysosome-Associated Membrane Protein 1; CD107 Antigen-Like Family Member A; CD107a; LAMP1
AA Sequence

AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM

Molecular Weight

90-130 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LAMP1/CD107a Protein, Human (HEK293, Fc)
Cat. No.:
HY-P7779
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