KREMEN1 Protein, Human (HEK293, Fc)

KREMEN1 Protein, Human (HEK293, Fc) is the recombinant human-derived KREMEN1 protein, expressed by HEK293, with C-hFc tag.

KREMEN1 is a receptor for Dickkopf proteins that cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6. In the absence of DKK1, it potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane. KREMEN1 can also trigger apoptosis in a Wnt-independent manner, an activity inhibited upon DKK1 binding. It plays a role in limb development by attenuating Wnt signaling for proper limb patterning and negatively regulates bone formation. Additionally, KREMEN1 modulates cell fate decisions in the developing cochlea, inhibiting hair cell fate specification.

Human

HEK293

C-hFc

Q96MU8-1 (R2-T392)

83999

Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.