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  3. Hydrolases (EC 3)
  4. Kras4A Protein, Human (Active, Biotinylated, His-Avi)

Kras4A Protein, Human (Active, Biotinylated, His-Avi)

Cat. No.: HY-P704771
Handling Instructions Technical Support

The Kras4B protein interacts specifically with GPR31, dependent on farnesylation. This binding suggests a regulatory role for Kras4B in association with GPR31, emphasizing the importance of the farnesylation process. Comprehensive exploration into the molecular details of this interaction is crucial to understand the precise mechanisms and functional implications in cellular processes or signaling pathways. Kras4A Protein, Human (Active, Biotinylated, His-Avi) is the recombinant human-derived Kras4A protein, expressed by E. coli, with N-His and N-Avi labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The Kras4B protein interacts specifically with GPR31, dependent on farnesylation. This binding suggests a regulatory role for Kras4B in association with GPR31, emphasizing the importance of the farnesylation process. Comprehensive exploration into the molecular details of this interaction is crucial to understand the precise mechanisms and functional implications in cellular processes or signaling pathways. Kras4A Protein, Human (Active, Biotinylated, His-Avi) is the recombinant human-derived Kras4A protein, expressed by E. coli, with N-His and N-Avi labeled tag.

Background

The Kras4B protein establishes a specific interaction with GPR31, and this binding is contingent on farnesylation. This interaction underscores the potential regulatory role of Kras4B in conjunction with GPR31, suggesting a dependence on the farnesylation process. Further exploration into the molecular intricacies of this interaction is essential to unravel the precise mechanisms and functional implications associated with the interplay between Kras4B and GPR31 in cellular processes or signaling pathways.

Species

Human

Source

E. coli

Tag

N-His;N-Avi

Accession

P01116-1 (T2-C186)

Gene ID

3845

Molecular Construction
N-term
His
Avi
(T2-C186)
Accession # P01116-1
C-term
Protein Length

Partial

Synonyms
Ki-Ras; c-K-ras; KRAS2; RASK2; CFC2; KRAS; GTPase KRas; 2A; K-Ras4A; Ki-Ras; c-K-ras
Predicted Molecular Mass
21 kDa
Molecular Weight

Approximately 27 kDa, based on SDS-PAGE under reducing conditions.

Structure/Form
Monomer
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Kras4A Protein, Human (Active, Biotinylated, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Kras4A Protein, Human (Active, Biotinylated, His-Avi)
Cat. No.:
HY-P704771
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