Jagged-2/JAG2 Protein, Human (CHO, hFc)

JAG2 is one of the NOTCH ligands. JAG2 modulates the sensitivity of CRC cells to chemotherapeutic agents through p21. JAG2 was involved in the regulation of migration and invasion independent of the canonical Notch signaling pathway. JAG2 also promoted the migration and invasion of colon cancer cells in a non-EMT pathway^[1][2]. Jagged-2/JAG2 Protein, Human (CHO, hFc) is the recombinant human-derived Jagged-2/JAG2 protein, expressed by CHO , with C-hFc labeled tag.

Modulation of JAG2 expression significantly affected in vivo tumorigenicity of CRC cell lines. Moreover, knockdown of JAG2 sensitized CRC cells to chemotherapeutic agents, while ectopic expression of JAG2 increased chemoresistance of the CRC cells. Significant down-regulation of p21 was observed in JAG2-knockdown cells. NOTCH signaling consists of four receptors (NOTCH1-NOTCH4), three delta-like ligands (DLL1, DLL3, and DLL4), and two serrate-like ligands (JAG1 and JAG2). Knockdown or pharmacological inhibition of β-catenin reduces JAG2 levels in the CRC cell lines^[1].The human JAG2 gene is located on chromosome 14q32 and consists of 26 exons which encode mRNA variants with 5825 bp and 5721 bp in length. The protein encoded by this gene contains a signal peptide, an extracellular DSL region, 16 EGF-like repeats, a cysteine-rich region, a transmembrane region, and a short cytoplasmic region of 132 amino acids^[2].

Human

CHO

C-hFc

AAB84215 (M27-L1082)

3714  [NCBI]

Approximately 140 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.