Jagged-1/JAG1 Protein, Rat (HEK293, His)

Jagged-1/JAG1 protein is a ligand of multiple Notch receptors, mediates Notch signaling, affects cell fate decisions during hematopoiesis and enhances angiogenesis. It functions throughout mammalian cardiovascular development, regulates myoblast differentiation, and participates in the complex Notch signaling network through interactions with NOTCH1, NOTCH2, and NOTCH3. Jagged-1/JAG1 Protein, Rat (HEK293, His) is the recombinant rat-derived Jagged-1/JAG1 protein, expressed by HEK293.

Jagged-1 (JAG1) serves as a ligand for multiple Notch receptors, playing a pivotal role in mediating Notch signaling. It is implicated in cell-fate decisions during hematopoiesis and demonstrates the capacity to enhance fibroblast growth factor-induced angiogenesis in vitro. JAG1's involvement spans various stages of mammalian cardiovascular development, highlighting its significance in these processes. Additionally, it exerts an inhibitory effect on myoblast differentiation, suggesting its regulatory role in diverse cellular functions. Through interactions with NOTCH1, NOTCH2, and NOTCH3, JAG1 contributes to the intricate network of Notch signaling, underscoring its multifaceted role in cellular responses and developmental pathways.

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.

Rat

HEK293

C-6*His

Q63722 (S32-D1067, with substitutions GGARN56-60AE and DR63-64TLVRPY)

29146

Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.