Integrin alpha IV beta 1 Protein, Human (HEK293, His)

Measured by its binding ability in a functional ELISA. Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free.The bioactivity was comparable to standard batch.

Human

HEK293

C-His

P13612-1 (Y34-T977)& P05556-1 (Q21-D728)

3676&3688

Approximately 60-75 kDa (ITGA4), 110-140 kDa (ITGA1), based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.