GSDME Protein, Human (His)

GSDME Protein, Human (His) is the recombinant human-derived GSDME protein, expressed by E. coli, with N-6*His tag.

DFNA5 is a precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis. Its N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores upon cleavage, triggering pyroptosis. The protein can be cleaved by CASP3 or granzyme B (GZMB), promoting the switch from apoptosis to pyroptosis or granzyme-mediated pyroptosis, respectively. After cleavage, DFNA5 translocates to the plasma membrane, forms pores (10-15 nm in diameter), and releases mature interleukins (e.g., IL1B and IL16) to induce pyroptosis. It also binds to bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. CASP3-mediated cleavage enables DFNA5 to convert TNF- or chemotherapy-induced apoptosis into pyroptosis. Additionally, DFNA5 mediates secondary necrosis downstream of mitochondrial apoptosis and viral infections, exhibits bactericidal activity, and suppresses tumors via GZMB-triggered pyroptosis in NK cell targets. Similar roles include: involvement in p53/TP53-regulated DNA damage response. Microbial infection-related function: DFNA5 promotes maternal placental pyroptosis during Zika virus infection, contributing to adverse fetal outcomes.

Human

E. coli

N-6*His

O60443-1 (M1-S496)

1687

Full Length of Isoform-1

MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Approximately 60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.