FITC-Labeled CD4 Protein, Human (HEK293, His)

The CD4 protein is an integral membrane glycoprotein that plays a key role in immune responses. In T cells, it serves as a coreceptor for MHC class II molecules, interacting with T cell receptors and initiating intracellular signaling pathways. FITC-Labeled CD4 Protein, Human (HEK293, His) is the recombinant human-derived FITC-Labeled CD4 protein, expressed by HEK293 , with C-His labeled tag. The total length of FITC-Labeled CD4 Protein, Human (HEK293, His) is 365 a.a., with molecular weight of 45-55 kDa.

The CD4 protein, an integral membrane glycoprotein, assumes a crucial role in immune responses, undertaking diverse functions against both external and internal challenges. In T-cells, its primary function is as a coreceptor for the MHC class II molecule:peptide complex, where class II peptides originate from extracellular proteins, while class I peptides are derived from cytosolic proteins. CD4 interacts concurrently with the T-cell receptor (TCR) and the MHC class II presented by antigen-presenting cells (APCs), leading to the recruitment of the Src kinase LCK to the vicinity of the TCR-CD3 complex. Subsequently, LCK initiates various intracellular signaling pathways by phosphorylating diverse substrates, ultimately resulting in lymphokine production, enhanced motility, adhesion, and the activation of T-helper cells. In other cell types such as macrophages or NK cells, CD4 contributes to differentiation/activation, cytokine expression, and cell migration through a TCR/LCK-independent pathway. Additionally, it plays a pivotal role in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. Notably, CD4 acts as the primary receptor for human immunodeficiency virus-1 (HIV-1), with its down-regulation facilitated by HIV-1 Vpu, and it also serves as a receptor for Human Herpes virus 7/HHV-7.

Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4 His with a linear range of 0.01-0.156 μg/mL.

Human

HEK293

C-His

P01730 (K26-W390)

920  [NCBI]

45-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 1 year from date of receipt, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.